| The pss4 gene from Rhizobium leguminosarum by viciae VF39: cloning, sequence and the possible role in polysaccharide production and nodule formation. | |
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MedLine Citation:
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PMID: 7959035 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Tn5 insertion into the genome of Rhizobium leguminosarum bv viciae VF39, resulting in non-mucoid growth and formation of non-N2-fixing nodule-like structures on Vicia faba plants, was mapped within a 1.4-kb EcoRV-SacI fragment. Nucleotide sequence analysis revealed an ORF (pss4) of 263 amino acids (aa). Three transcription start points (tsp) were determined. Two of them were localized upstream from the first GTG codon; the third tsp was mapped in front of the second putative start codon (GTG) corresponding to Val64 of the Pss4 aa sequence. The expression of pss4 in a T7 RNA polymerase/promoter system produced a single approx. 29-kDa protein. Pss4 reveals similarity to several proteins involved in polysaccharide biosynthesis in various Rhizobium species. A nearly complete homology was found with PssA from Rl biovar phaseoli 8002 [Borthakur et al., Mol. Gen. Genet. 213 (1988) 155-162], except that Pss4 has an additional 63 aa on its N terminus. |
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Authors:
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T V Ivashina; M I Khmelnitsky; M G Shlyapnikov; A A Kanapin; V N Ksenzenko |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Gene Volume: 150 ISSN: 0378-1119 ISO Abbreviation: Gene Publication Date: 1994 Dec |
Date Detail:
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Created Date: 1994-12-21 Completed Date: 1994-12-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7706761 Medline TA: Gene Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 111-6 Citation Subset: IM |
Affiliation:
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Institute of Biochemistry and Physiology of Microorganisms, Academy of Sciences, Pushchino, Moscow Region, Russia. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/M93042 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Bacterial Proteins / genetics Base Sequence Cloning, Molecular DNA Transposable Elements / genetics DNA, Bacterial Fabaceae / microbiology Genes, Bacterial* Membrane Proteins / genetics Molecular Sequence Data Nucleic Acid Conformation Plants, Medicinal Polysaccharides, Bacterial / biosynthesis RNA, Messenger / chemistry, genetics Regulatory Sequences, Nucleic Acid Rhizobium leguminosarum / genetics*, physiology Sequence Homology, Amino Acid Symbiosis Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/DNA Transposable Elements; 0/DNA, Bacterial; 0/EPSI polysaccharide; 0/Membrane Proteins; 0/Polysaccharides, Bacterial; 0/Pss4 protein, Rhizobium leguminosarum; 0/RNA, Messenger |
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