Document Detail


A proton nuclear magnetic resonance-based metabonomics study of metabolic profiling in immunoglobulin a nephropathy.
MedLine Citation:
PMID:  22522762     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: Immunoglobulin A nephropathy is the most common cause of chronic renal failure among primary glomerulonephritis patients. The ability to diagnose immunoglobulin A nephropathy remains poor. However, renal biopsy is an inconvenient, invasive, and painful examination, and no reliable biomarkers have been developed for use in routine patient evaluations. The aims of the present study were to identify immunoglobulin A nephropathy patients, to identify useful biomarkers of immunoglobulin A nephropathy and to establish a human immunoglobulin A nephropathy metabolic profile.
METHODS: Serum samples were collected from immunoglobulin A nephropathy patients who were not using immunosuppressants. A pilot study was undertaken to determine disease-specific metabolite biomarker profiles in three groups: healthy controls (N = 23), low-risk patients in whom immunoglobulin A nephropathy was confirmed as grades I-II by renal biopsy (N = 23), and high-risk patients with nephropathies of grades IV-V (N = 12). Serum samples were analyzed using proton nuclear magnetic resonance spectroscopy and by applying multivariate pattern recognition analysis for disease classification.
RESULTS: Compared with the healthy controls, both the low-risk and high-risk patients had higher levels of phenylalanine, myo-Inositol, lactate, L6 lipids ( = CH-CH2-CH = O), L5 lipids (-CH2-C = O), and L3 lipids (-CH2-CH2-C = O) as well as lower levels of β -glucose, α-glucose, valine, tyrosine, phosphocholine, lysine, isoleucine, glycerolphosphocholine, glycine, glutamine, glutamate, alanine, acetate, 3-hydroxybutyrate, and 1-methylhistidine.
CONCLUSIONS: These metabolites investigated in this study may serve as potential biomarkers of immunoglobulin A nephropathy. Point scoring of pattern recognition analysis was able to distinguish immunoglobulin A nephropathy patients from healthy controls. However, there were no obvious differences between the low-risk and high-risk groups in our research. These results offer new, sensitive and specific, noninvasive approaches that may be of great benefit to immunoglobulin A nephropathy patients by enabling earlier diagnosis.
Authors:
Weiguo Sui; Liping Li; Wenti Che; Zuo Guimai; Jiejing Chen; Wuxian Li; Yong Dai
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinics (São Paulo, Brazil)     Volume:  67     ISSN:  1980-5322     ISO Abbreviation:  Clinics (Sao Paulo)     Publication Date:  2012  
Date Detail:
Created Date:  2012-04-23     Completed Date:  2013-01-28     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101244734     Medline TA:  Clinics (Sao Paulo)     Country:  Brazil    
Other Details:
Languages:  eng     Pagination:  363-73     Citation Subset:  IM    
Affiliation:
Laboratory of Metabolic Diseases Research, Central Laboratory, 181st Hospital Guangxi, Guangxi Province, China. suiwg@163.com
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Biological Markers / analysis
Biopsy
Case-Control Studies
Discriminant Analysis
Female
Glomerulonephritis, IGA / diagnosis*,  metabolism,  pathology
Humans
Kidney / pathology
Least-Squares Analysis
Magnetic Resonance Spectroscopy / methods*
Male
Metabolomics / methods*
Protons
Sensitivity and Specificity
Young Adult
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Protons
Comments/Corrections

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