Document Detail

A proton-dependent zinc uptake in PC12 cells.
MedLine Citation:
PMID:  15895819     Owner:  NLM     Status:  MEDLINE    
Intracellular pH in pheochromocytoma (PC12) cells was manipulated by 'acid loading' the cells and the effect of such a change on radioactive zinc uptake was studied. It was found that zinc uptake was stimulated in cells loaded with protons without causing any measurable change in the intracellular pH. To confirm our assumption that the proton flux due to zinc entry is too small to be measured, we calculated the pH change that one would expect because of zinc influx. The intrinsic buffer capacity of PC12 cells was determined to be 8.03 mM/pH unit and was used in these calculations. It was found that at the five-minute incubation, zinc uptake occurring under our experimental conditions could cause a pH change of 0.000277 pH units per minute (assuming a 1:2 zinc:proton stoichiometry). This study adds a new dimension towards understanding the role played by intracellular pH in causing zinc entry into cells.
Rengarajan V Balaji; Robert A Colvin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neurochemical research     Volume:  30     ISSN:  0364-3190     ISO Abbreviation:  Neurochem. Res.     Publication Date:  2005 Feb 
Date Detail:
Created Date:  2005-05-17     Completed Date:  2005-06-08     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7613461     Medline TA:  Neurochem Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  171-6     Citation Subset:  IM    
Program in Neuroscience, Department of Biological Sciences, Ohio University, Athens, OH-45701, USA.
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MeSH Terms
Cell Membrane / metabolism
Hydrogen-Ion Concentration
Naphthols / diagnostic use
PC12 Cells
Rhodamines / diagnostic use
Sodium-Hydrogen Antiporter / metabolism
Spectrometry, Fluorescence
Zinc / metabolism*
Zinc Radioisotopes / diagnostic use
Reg. No./Substance:
0/Benzopyrans; 0/Buffers; 0/Naphthols; 0/Protons; 0/Rhodamines; 0/Sodium-Hydrogen Antiporter; 0/Zinc Radioisotopes; 0/seminaphthorhodaminefluoride; 7440-66-6/Zinc

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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