| A protocol for the cryoconservation of breeds by low-cost emergency cell banks - a pilot study. | |
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MedLine Citation:
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PMID: 22444957 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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A protocol was developed for collection and storage of somatic cell samples under adverse conditions with little infrastructure, for somatic-cell banks as a backup for endangered livestock breeds. The procedure, which is uniform across species, includes sample collection with ear taggers with an integrated tag/vial system, and recording of global positioning system (GPS) coordinates, digital images and breed information. In a pilot study, the procedures were tested on six local Vietnamese populations of pigs, sheep and goats. Initial investment was around €3000, while the total variable cost for sampling one breed with 25 females and 25 males was less than €1000. With support from local organisations, the sampling of six breeds with 300 animals was carried out in 2 months. The protocol and the complete workflow for setting up a somatic-cell bank, together with data collection, are described. The procedure has proved practicable and exceedingly cheap relative to the cryopreservation of semen or embryos. |
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Authors:
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E Groeneveld; Nguyen Huu Tinh; W Kues; Nguyen Thi Vien |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Animal : an international journal of animal bioscience Volume: 2 ISSN: 1751-7311 ISO Abbreviation: Animal Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2012-03-26 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101303270 Medline TA: Animal Country: England |
Other Details:
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Languages: eng Pagination: 1-8 Citation Subset: - |
Affiliation:
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1Institute for Animal Breeding, Federal Agricultural Research Centre, Mariensee, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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