Document Detail


A proteomics approach identifies novel proteins involved in gravitropic signal transduction.
MedLine Citation:
PMID:  23281391     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PREMISE: Plant organs use gravity as a guide to direct their growth. And although gravitropism has been studied since the time of Darwin, the mechanisms of signal transduction, those that connect the biophysical stimulus perception and the biochemical events of the response, are still not understood.
METHODS: A quantitative proteomics approach was used to identify key proteins during the early events of gravitropism. Plants were subjected to a gravity persistent signal (GPS) treatment, and proteins were extracted from the inflorescence stem at early time points after stimulation. Proteins were labeled with isobaric tags for relative and absolute quantification (iTRAQ) reagents. Proteins were identified and quantified as a single step using tandem mass-spectrometry (MS/MS). For two of the proteins identified, mutants with T-DNA inserts in the corresponding genes were evaluated for gravitropic phenotypes.
KEY RESULTS: A total of 82 proteins showed significant differential quantification between treatment and controls. Proteins were categorized into functional groups based on gene ontology terms and filtered using groups thought to be involved in the signaling events of gravitropism. For two of the proteins selected, GSTF9 and HSP81-2, knockout mutations resulted in defects in root skewing, waving, and curvature as well as in the GPS response of inflorescence stems.
CONCLUSION: Combining a proteomics approach with the GPS response, 82 novel proteins were identified to be involved in the early events of gravitropic signal transduction. As early as 2 and 4 min after a gravistimulation, significant changes occur in protein abundance. The approach was validated through the analysis of mutants exhibiting altered gravitropic responses.
Authors:
Craig A Schenck; Vijayanand Nadella; Shannon L Clay; Jessica Lindner; Zachary Abrams; Sarah E Wyatt
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-01-01
Journal Detail:
Title:  American journal of botany     Volume:  100     ISSN:  1537-2197     ISO Abbreviation:  Am. J. Bot.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-03     Completed Date:  2013-06-11     Revised Date:  2013-11-06    
Medline Journal Info:
Nlm Unique ID:  0370467     Medline TA:  Am J Bot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  194-202     Citation Subset:  IM    
Affiliation:
Department of Environmental and Plant Biology, Ohio University, Athens, Ohio 45701, USA.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / genetics,  physiology*
Arabidopsis Proteins / metabolism*
DNA Mutational Analysis
Gravitropism / physiology*
Kinetics
Molecular Sequence Annotation
Mutation / genetics
Phenotype
Plant Roots / physiology
Proteomics / methods*
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction*
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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