| A proteomic snapshot of breast cancer cell cycle: The G1/S transition point. | |
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MedLine Citation:
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PMID: 23152136 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The biological processes that unfold during the G1-phase of the cell cycle are dependent on extracellular mitogenic factors which signal the cell to enter a state of quiescence, or commit to a cell cycle round by passing the restriction point (R-point) and enter the S-phase. Unlike normal cells, cancer cells evolved the ability to evade the R-point and continue through the cell cycle even in the presence of extensive DNA damage or absence of mitogenic signals. The purpose of this study was to perform a quantitative proteomic evaluation of the biological processes that are responsible for driving MCF-7 breast cancer cells into division even when molecular checkpoints such as the G1/S R-point are in place. Nuclear and cytoplasmic fractions of the G1 and S cell cycle phases were analyzed by LC-MS/MS to result in the confident identification of >2700 proteins. Statistical evaluation of the normalized data resulted in the selection of proteins that displayed ≥2-fold change in spectral counts in each cell state. Pathway mapping, functional annotation clustering and protein interaction network analysis revealed that the top-scoring clusters that could play a role in overriding the G1/S transition point included DNA damage response, chromatin remodeling, transcription/translation regulation and signaling proteins. |
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Authors:
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Milagros J Tenga; Iulia M Lazar |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-14 |
Journal Detail:
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Title: Proteomics Volume: - ISSN: 1615-9861 ISO Abbreviation: Proteomics Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-15 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101092707 Medline TA: Proteomics Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Affiliation:
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Department of Biological Sciences, Virginia Polytechnic Institute and State University, 1981 Kraft Drive, Blacksburg, VA, 246021. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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