Document Detail


A proteomic approach to analysis of antimicrobial activity in marsupial pouch secretions.
MedLine Citation:
PMID:  17582494     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have documented the antimicrobial activity of pouch secretions of the tammar wallaby, Macropus eugenii, over the period leading up to birth and after birth of the young animal. This activity was greatest against the Gram-negative Escherichia coli and highest at the time of birth. Fractionation of the pouch secretions showed that activity at different times over the reproductive periods was associated with different molecular-weight (MW) components, with compounds in the range up to 50 kDa active immediately prior to and at the time of birth. Proteomic analysis using 1 and 2DE PAGE and LC-MS/MS identified the major components of the pouch secretions at these times, at a range of pI's and MWs. The majority of high-confidence identifications, at a wide range of pI's and MW, were beta-lactoglobulin, a known component of marsupial milk. We subsequently conducted a proteomic analysis of mammary gland secretions and digest products from the gut of the young animal, using 2DE PAGE and MALDI MS/MS, to confirm its source and compare it with the observed MW and pI's of beta-lactoglobulin. Although we did not directly identify an effector molecule responsible for antimicrobial activity, these results lead us to propose that beta-lactoglobulin plays a role in the protection of the young marsupial, a role previously thought to be primarily due to specific secretions from the epithelial surface of the pouch.
Authors:
Kiran Ambatipudi; Janice Joss; Mark Raftery; Elizabeth Deane
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Publication Detail:
Type:  Journal Article     Date:  2007-06-04
Journal Detail:
Title:  Developmental and comparative immunology     Volume:  32     ISSN:  0145-305X     ISO Abbreviation:  Dev. Comp. Immunol.     Publication Date:  2008  
Date Detail:
Created Date:  2007-12-06     Completed Date:  2008-02-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7708205     Medline TA:  Dev Comp Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  108-20     Citation Subset:  IM    
Affiliation:
Department of Biological Sciences, Division of Environmental and Life Sciences, Macquarie University, NSW 2109, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Suckling / immunology
Anti-Bacterial Agents / isolation & purification*,  pharmacology
Escherichia coli / drug effects
Female
Lactoglobulins / isolation & purification*,  pharmacology
Macropodidae* / immunology
Mammary Glands, Animal / chemistry*,  immunology
Mass Spectrometry
Milk / chemistry*,  immunology
Peptides / isolation & purification*,  pharmacology
Proteomics*
Staphylococcus aureus / drug effects
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Lactoglobulins; 0/Peptides; 0/dermcidin

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