| C/EBP proteins activate transcription from the human immunodeficiency virus type 1 long terminal repeat in macrophages/monocytes. | |
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MedLine Citation:
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PMID: 7636977 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Three binding sites for C/EBP proteins are found in the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) (V. M. Tesmer, A. Rajadhyaksha, J. Babin, and M. Bina, Proc. Natl. Acad. Sci. USA 90:7298-7302, 1993). We have determined the functional role of C/EBP proteins and C/EBP sites in regulating transcription from the HIV-1 LTR in monocytes/macrophages. Inhibition of endogenous C/EBP proteins, using either an excess of C/EBP binding sites or a trans-dominant negative inhibitor, demonstrated that C/EBP proteins are required for basal and activated levels of HIV-1 LTR transcription in the promonocytic cell line U937. Northern (RNA) blots and binding assays showed that NF-IL6 is the only known C/EBP family member which is increased when U937 cells are activated. Mutational analyses of the HIV-1 LTR showed that one C/EBP site is required for normal LTR transcription both before and after cellular activation and that the two 3' C/EBP sites are functionally equivalent. However, transcription from crippled HIV-1 LTRs lacking C/EBP sites can still be induced following activation of U937 cells. Several models are suggested for how elevated NF-IL6 may participate in an autostimulatory loop involving HIV infection, macrophage activation, cytokine expression, and HIV replication. |
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Authors:
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A J Henderson; X Zou; K L Calame |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 69 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1995 Sep |
Date Detail:
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Created Date: 1995-09-14 Completed Date: 1995-09-14 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 5337-44 Citation Subset: IM; X |
Affiliation:
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Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence Binding Sites CCAAT-Enhancer-Binding Proteins Cell Differentiation Cell Line Chloramphenicol O-Acetyltransferase / biosynthesis DNA Primers DNA-Binding Proteins / metabolism* Gene Expression Gene Expression Regulation, Viral* HIV Long Terminal Repeat* HIV-1 / genetics, metabolism* Humans Lipopolysaccharides / pharmacology Luciferases / biosynthesis Macrophages / metabolism, virology Molecular Sequence Data Monocytes / metabolism, virology Nuclear Proteins / metabolism* Polymerase Chain Reaction Recombinant Proteins / biosynthesis Restriction Mapping Transcription Factors / metabolism Transcription, Genetic Transfection |
| Grant Support | |
ID/Acronym/Agency:
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GM29361/GM/NIGMS NIH HHS; GM44300/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CCAAT-Enhancer-Binding Proteins; 0/DNA Primers; 0/DNA-Binding Proteins; 0/Lipopolysaccharides; 0/Nuclear Proteins; 0/Recombinant Proteins; 0/Transcription Factors; EC 1.13.12.-/Luciferases; EC 2.3.1.28/Chloramphenicol O-Acetyltransferase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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