Document Detail


C/EBP proteins activate transcription from the human immunodeficiency virus type 1 long terminal repeat in macrophages/monocytes.
MedLine Citation:
PMID:  7636977     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Three binding sites for C/EBP proteins are found in the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR) (V. M. Tesmer, A. Rajadhyaksha, J. Babin, and M. Bina, Proc. Natl. Acad. Sci. USA 90:7298-7302, 1993). We have determined the functional role of C/EBP proteins and C/EBP sites in regulating transcription from the HIV-1 LTR in monocytes/macrophages. Inhibition of endogenous C/EBP proteins, using either an excess of C/EBP binding sites or a trans-dominant negative inhibitor, demonstrated that C/EBP proteins are required for basal and activated levels of HIV-1 LTR transcription in the promonocytic cell line U937. Northern (RNA) blots and binding assays showed that NF-IL6 is the only known C/EBP family member which is increased when U937 cells are activated. Mutational analyses of the HIV-1 LTR showed that one C/EBP site is required for normal LTR transcription both before and after cellular activation and that the two 3' C/EBP sites are functionally equivalent. However, transcription from crippled HIV-1 LTRs lacking C/EBP sites can still be induced following activation of U937 cells. Several models are suggested for how elevated NF-IL6 may participate in an autostimulatory loop involving HIV infection, macrophage activation, cytokine expression, and HIV replication.
Authors:
A J Henderson; X Zou; K L Calame
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  69     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1995 Sep 
Date Detail:
Created Date:  1995-09-14     Completed Date:  1995-09-14     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  5337-44     Citation Subset:  IM; X    
Affiliation:
Department of Microbiology, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Binding Sites
CCAAT-Enhancer-Binding Proteins
Cell Differentiation
Cell Line
Chloramphenicol O-Acetyltransferase / biosynthesis
DNA Primers
DNA-Binding Proteins / metabolism*
Gene Expression
Gene Expression Regulation, Viral*
HIV Long Terminal Repeat*
HIV-1 / genetics,  metabolism*
Humans
Lipopolysaccharides / pharmacology
Luciferases / biosynthesis
Macrophages / metabolism,  virology
Molecular Sequence Data
Monocytes / metabolism,  virology
Nuclear Proteins / metabolism*
Polymerase Chain Reaction
Recombinant Proteins / biosynthesis
Restriction Mapping
Transcription Factors / metabolism
Transcription, Genetic
Transfection
Grant Support
ID/Acronym/Agency:
GM29361/GM/NIGMS NIH HHS; GM44300/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/CCAAT-Enhancer-Binding Proteins; 0/DNA Primers; 0/DNA-Binding Proteins; 0/Lipopolysaccharides; 0/Nuclear Proteins; 0/Recombinant Proteins; 0/Transcription Factors; EC 1.13.12.-/Luciferases; EC 2.3.1.28/Chloramphenicol O-Acetyltransferase
Comments/Corrections

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