Document Detail


The protein kinase CaSch9p is required for the cell growth, filamentation and virulence in the human fungal pathogen Candida albicans.
MedLine Citation:
PMID:  20345900     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The target of rapamycin complex 1 (TORC1) is the central controller of growth in eukaryotic cells. As one of the downstream targets of TORC1, the protein kinase ScSch9p plays multiple roles in stress resistance, longevity and nutrient sensing in Saccharomyces cerevisiae. In this study, we demonstrate that Candida albicans cells with CaSCH9 deleted have reduced cell sizes and show a delayed log-phase growth. In addition, deletion of CaSCH9 renders C. albicans cells sensitive to rapamycin, caffeine and sodium dodecyl sulfate. Similar to ScSCH9, deletion of CaSCH9 also causes C. albicans cells to become sensitive to cations, but does not lead to a defect in the utilization of galactose. Furthermore, deletion of CaSCH9 affects the filamentation of C. albicans cells and attenuates the virulence in a mouse mode of systemic candidiasis. Therefore, CaSch9p is an important regulator for the cell growth, filamentation and virulence of this human fungal pathogen.
Authors:
Wei Liu; Jingwen Zhao; Xichuan Li; Yunxiang Li; Linghuo Jiang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-05
Journal Detail:
Title:  FEMS yeast research     Volume:  10     ISSN:  1567-1364     ISO Abbreviation:  FEMS Yeast Res.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-18     Completed Date:  2010-09-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101085384     Medline TA:  FEMS Yeast Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  462-70     Citation Subset:  IM    
Affiliation:
Department of Molecular and Cellular Pharmacology, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antifungal Agents / pharmacology
Caffeine / pharmacology
Candida albicans / growth & development*,  pathogenicity*
Candidiasis / microbiology
Fungal Proteins / genetics,  physiology*
Galactose / metabolism
Gene Deletion
Male
Mice
Mice, Inbred BALB C
Protein Kinases / deficiency,  physiology*
Sirolimus / pharmacology
Sodium Dodecyl Sulfate / pharmacology
Survival Analysis
Virulence
Virulence Factors / deficiency,  physiology*
Chemical
Reg. No./Substance:
0/Antifungal Agents; 0/Fungal Proteins; 0/Virulence Factors; 151-21-3/Sodium Dodecyl Sulfate; 26566-61-0/Galactose; 53123-88-9/Sirolimus; 58-08-2/Caffeine; EC 2.7.-/Protein Kinases; EC 2.7.1.-/SCH9 protein kinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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