Document Detail

The protein kinase C activator, phorbol ester, elicits disparate functional responses in androgen-sensitive and androgen-independent human prostatic cancer cells.
MedLine Citation:
PMID:  9514905     Owner:  NLM     Status:  MEDLINE    
The protein kinase C (PKC) activator 12-O-tetradecanoyl-phorbol-13-acetate (TPA) activated cell death in androgen-sensitive LNCaP cells but not in androgen-independent DU-145 or PC-3 cells, whose growth was significantly decreased by PKC inhibitors staurosporine and H7. All cell lines had similar levels of total PKC activities which, however, differed on their dependency on Ca2+ ions and lipid and were regulated differently by TPA. Furthermore, expression of the immediate early genes c-fos and c-jun was up-regulated by TPA only in LNCaP and DU-145 cells, whereas PC-3 cells failed to express c-fos mRNA. The regulation of the c-myc mRNA by TPA correlated inversely with activation of cell death being down-regulated in LNCaP cells, and slightly increased in the androgen-independent cell lines. These results suggest that the PKC signal transduction pathway functions differently in androgen-sensitive and insensitive prostatic cells.
P Henttu; P Vihko
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  244     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-04-09     Completed Date:  1998-04-09     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  167-71     Citation Subset:  IM    
Biocenter Oulu, University of Oulu, Finland.
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MeSH Terms
Androgens / physiology*
Cell Division / drug effects,  genetics
Enzyme Activation / drug effects
Epidermal Growth Factor / pharmacology
Gene Expression Regulation, Neoplastic / drug effects
Genes, Immediate-Early / drug effects
Genes, fos / drug effects
Genes, jun / drug effects
Genes, myc / drug effects
Prostatic Neoplasms / enzymology*,  genetics,  pathology*
Protein Kinase C / antagonists & inhibitors,  metabolism*
Tetradecanoylphorbol Acetate / pharmacology*
Tumor Cells, Cultured
Reg. No./Substance:
0/Androgens; 16561-29-8/Tetradecanoylphorbol Acetate; 62229-50-9/Epidermal Growth Factor; EC Kinase C

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