Document Detail


A protein-free diet alters small intestinal architecture and digestive enzyme activities in rats.
MedLine Citation:
PMID:  16438914     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The consequences of feeding a protein-free (PF) diet, as compared to casein, on gut characteristics were studied in slightly energy-restricted rats fed similar amounts of feed for 10 d. The weight and pH of fresh digesta in the stomach were lower (P = 0.045 and P = 0.016). However, the weight of fresh digesta in the other segments and gut tissue weight were not significantly affected by the diet (P > 0.05). Small intestinal crypt depth, width and area were reduced by 13, 23 and 37%, respectively (P = 0.011, P = 0.004 and P = 0.001), and villus width tended to be smaller (P = 0.057), with the PF diet. Villus height to crypt depth ratio was also lower with the PF diet in the duodenum and ileum, respectively (P < 0.05). Finally, the specific activities of alkaline phosphatase and aminopeptidase N were reduced by 36 to 38% at different sites of the small intestine in the rats fed the PF diet (P < 0.05). In conclusion, chronic consumption of a protein-free diet altered the intestinal villus-crypt architecture and enzyme activities in rats.
Authors:
Carlos Alexander Montoya; Pascal Leterme; Jean-Paul Lalles
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Reproduction, nutrition, development     Volume:  46     ISSN:  0926-5287     ISO Abbreviation:  Reprod. Nutr. Dev.     Publication Date:    2006 Jan-Feb
Date Detail:
Created Date:  2006-01-27     Completed Date:  2006-03-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8913069     Medline TA:  Reprod Nutr Dev     Country:  France    
Other Details:
Languages:  eng     Pagination:  49-56     Citation Subset:  IM    
Affiliation:
Universidad Nacional de Colombia, Departamento de Produccion Animal, Carrera 32 Chapinero, Palmira (Valle), Colombia.
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MeSH Terms
Descriptor/Qualifier:
Alkaline Phosphatase / metabolism
Animals
Antigens, CD13 / metabolism
Caseins / administration & dosage
Diet, Protein-Restricted*
Dietary Proteins / administration & dosage*
Female
Hydrogen-Ion Concentration
Intestinal Mucosa / anatomy & histology,  drug effects*,  enzymology,  growth & development
Intestine, Small* / anatomy & histology,  drug effects,  enzymology,  growth & development
Organ Size
Random Allocation
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Caseins; 0/Dietary Proteins; EC 3.1.3.1/Alkaline Phosphatase; EC 3.4.11.2/Antigens, CD13

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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