| A protein-based oxygen biosensor for high-throughput monitoring of cell growth and cell viability. | |
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MedLine Citation:
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PMID: 19084497 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Fluorescently labeled hemocyanin has been previously proposed as an oxygen sensor. In this study, we explored the efficacy of this biosensor for monitoring the biological oxygen consumption of bacteria and its use in testing bacterial cell growth and viability of Escherichia coli, Pseudomonas aeruginosa, Paracoccus denitrificans, and Staphylococcus simulans. Using a microwell plate, the time courses for the complete deoxygenation of samples with different initial concentrations of cells were obtained and the doubling times were extracted. The applicability of our fluorescence-based cell growth assay as an antibacterial drug screening method was also explored. The results provide a proof-of-principle for a simple, quantitative, and sensitive method for high-throughput monitoring of prokaryotic cell growth and antibiotic susceptibility screening. |
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Authors:
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Maria Strianese; Gerhild Zauner; Armand W J W Tepper; Luigi Bubacco; Eefjan Breukink; Thijs J Aartsma; Gerard W Canters; Leandro C Tabares |
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Publication Detail:
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Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2008-11-21 |
Journal Detail:
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Title: Analytical biochemistry Volume: 385 ISSN: 1096-0309 ISO Abbreviation: Anal. Biochem. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-19 Completed Date: 2009-03-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: United States |
Other Details:
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Languages: eng Pagination: 242-8 Citation Subset: IM |
Affiliation:
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Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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pharmacology Bacteria / cytology*, growth & development Biosensing Techniques / methods* Escherichia coli / cytology, growth & development Hemocyanin Microbial Sensitivity Tests Oxygen / analysis* Paracoccus denitrificans / cytology, growth & development Pseudomonas aeruginosa / cytology, growth & development Staphylococcus / cytology, growth & development |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 7782-44-7/Oxygen; 9013-72-3/Hemocyanin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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