Document Detail


The protease resistant surface (PRS) glycoconjugate from Trypanosoma congolense has an inositol-acylated glycosylphosphatidylinositol anchor, containing a significant proportion of myristate at the sn-2 position.
MedLine Citation:
PMID:  20138924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In the tsetse fly, the surface of Trypanosoma congolense parasites is covered by a dense layer of glycosylphosphatidylinositol (GPI)-anchored molecules. These include EPGENGT procyclin and protease resistant surface molecule (PRS), as well as congolense epimastigote-specific protein, CESP, and glutamic acid- and alanine-rich protein (GARP). The GPI structures of EPGENGT and GARP have been partially elucidated, but very little is known about PRS. We now purified PRS and analyzed its GPI lipid structure and carbohydrate composition using mass spectrometry. We found that unlike EPGENGT and GARP, the GPI anchor of PRS is unusually composed of inositol-acylated diacyl-phosphatidylinositols, including species containing either myristic or oleic acid at the sn-2 position of the glycerol backbone. This is the first identification of a tri-acylated GPI anchor containing myristate in procyclic form trypanosomes. In addition, we found that PRS is highly rich in galactose and sialic acid residues, suggesting that it may represent a major acceptor of the parasite trans-sialidase.
Authors:
Eva Greganova; Peter Bütikofer; Alvaro Acosta-Serrano
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-06
Journal Detail:
Title:  Molecular and biochemical parasitology     Volume:  171     ISSN:  1872-9428     ISO Abbreviation:  Mol. Biochem. Parasitol.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-15     Completed Date:  2010-05-25     Revised Date:  2014-04-16    
Medline Journal Info:
Nlm Unique ID:  8006324     Medline TA:  Mol Biochem Parasitol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  50-4     Citation Subset:  IM    
Copyright Information:
(c) 2010 Elsevier B.V. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Cell Membrane / chemistry*
Galactose / analysis
Glycoconjugates / chemistry*,  isolation & purification
Glycosylphosphatidylinositols / analysis*
Mass Spectrometry
Myristic Acid / analysis*
N-Acetylneuraminic Acid / analysis
Oleic Acid / analysis
Protozoan Proteins / chemistry*,  isolation & purification
Trypanosoma congolense / chemistry*,  physiology*
Grant Support
ID/Acronym/Agency:
070937//Wellcome Trust; //Wellcome Trust
Chemical
Reg. No./Substance:
0/Glycoconjugates; 0/Glycosylphosphatidylinositols; 0/Protozoan Proteins; 0I3V7S25AW/Myristic Acid; 2UMI9U37CP/Oleic Acid; GZP2782OP0/N-Acetylneuraminic Acid; X2RN3Q8DNE/Galactose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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