| The protease resistant surface (PRS) glycoconjugate from Trypanosoma congolense has an inositol-acylated glycosylphosphatidylinositol anchor, containing a significant proportion of myristate at the sn-2 position. | |
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MedLine Citation:
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PMID: 20138924 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In the tsetse fly, the surface of Trypanosoma congolense parasites is covered by a dense layer of glycosylphosphatidylinositol (GPI)-anchored molecules. These include EPGENGT procyclin and protease resistant surface molecule (PRS), as well as congolense epimastigote-specific protein, CESP, and glutamic acid- and alanine-rich protein (GARP). The GPI structures of EPGENGT and GARP have been partially elucidated, but very little is known about PRS. We now purified PRS and analyzed its GPI lipid structure and carbohydrate composition using mass spectrometry. We found that unlike EPGENGT and GARP, the GPI anchor of PRS is unusually composed of inositol-acylated diacyl-phosphatidylinositols, including species containing either myristic or oleic acid at the sn-2 position of the glycerol backbone. This is the first identification of a tri-acylated GPI anchor containing myristate in procyclic form trypanosomes. In addition, we found that PRS is highly rich in galactose and sialic acid residues, suggesting that it may represent a major acceptor of the parasite trans-sialidase. |
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Authors:
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Eva Greganova; Peter B?tikofer; Alvaro Acosta-Serrano |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-06 |
Journal Detail:
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Title: Molecular and biochemical parasitology Volume: 171 ISSN: 1872-9428 ISO Abbreviation: Mol. Biochem. Parasitol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-03-15 Completed Date: 2010-05-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8006324 Medline TA: Mol Biochem Parasitol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 50-4 Citation Subset: IM |
Copyright Information:
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(c) 2010 Elsevier B.V. All rights reserved. |
Affiliation:
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Institute of Biochemistry and Molecular Medicine, University of Bern, B?hlstrasse 28, 3012 Bern, Switzerland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Membrane
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chemistry* Galactose / analysis Glycoconjugates / chemistry*, isolation & purification Glycosylphosphatidylinositols / analysis* Mass Spectrometry Myristic Acid / analysis* N-Acetylneuraminic Acid / analysis Oleic Acid / analysis Protozoan Proteins / chemistry*, isolation & purification Trypanosoma congolense / chemistry*, physiology* |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/Glycoconjugates; 0/Glycosylphosphatidylinositols; 0/Protozoan Proteins; 112-80-1/Oleic Acid; 131-48-6/N-Acetylneuraminic Acid; 26566-61-0/Galactose; 544-63-8/Myristic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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