| A prospective clinical study of Epstein-Barr virus and host interactions during acute infectious mononucleosis. | |
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MedLine Citation:
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PMID: 16206064 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Characterizing virus-host interactions during self-limited infectious mononucleosis could explain how Epstein-Barr virus (EBV) replication is normally controlled and provide insight into why certain immunocompromised patients fail to contain it. METHODS: University students had an average of 7 clinical and virologic evaluations during acute infectious mononucleosis. EBV was quantified in 697 samples of oral wash fluid, whole blood, peripheral blood mononuclear cells (PBMCs), and plasma by a real-time (TaqMan) polymerase chain reaction (qEBV) assay developed in our laboratory. RESULTS: Twenty of 25 subjects had serologically confirmed primary EBV infection. EBV was cleared from whole blood by a first-order process with a median half-life of 3 days, and its quantity was associated with severity of illness (r2=0.82). Oral shedding persisted at a median of >or=1x104 copies/mL for 32 weeks and was unrelated to severity of illness. Subjects with nonprimary EBV infection shed virus intermittently, and median quantities for all samples became undetectable within 4 weeks. CONCLUSIONS: Using a novel qEBV assay, we demonstrated that young adults with primary EBV infection rapidly cleared virus from blood but not from the oropharynx. High oral concentrations of EBV in asymptomatic persons who have resumed normal activities support the concept that infectious mononucleosis is most likely acquired by kissing. |
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Authors:
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Henry H Balfour; Carol J Holman; Kristin M Hokanson; Meghan M Lelonek; Jill E Giesbrecht; Dana R White; David O Schmeling; Chiu-Ho Webb; Winston Cavert; David H Wang; Richard C Brundage |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't Date: 2005-09-26 |
Journal Detail:
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Title: The Journal of infectious diseases Volume: 192 ISSN: 0022-1899 ISO Abbreviation: J. Infect. Dis. Publication Date: 2005 Nov |
Date Detail:
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Created Date: 2005-10-05 Completed Date: 2006-03-16 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0413675 Medline TA: J Infect Dis Country: United States |
Other Details:
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Languages: eng Pagination: 1505-12 Citation Subset: AIM; IM |
Affiliation:
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Department of Laboratory Medicine and Pathology, University of Minnesota, Fairview Clinical Virology Laboratory, Minneapolis 55455-0392, USA. balfo001@umn.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Antibodies, Viral / blood Biological Markers / analysis Disease Transmission, Infectious Epstein-Barr Virus Nuclear Antigens / genetics Female Genes, Viral / genetics Herpesvirus 4, Human / genetics, immunology, isolation & purification* Humans Infectious Mononucleosis / blood, pathology, transmission, virology* Male Oropharynx / virology Polymerase Chain Reaction Prospective Studies Time Factors Viral Load |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Viral; 0/Biological Markers; 0/EBV-encoded nuclear antigen 1; 0/Epstein-Barr Virus Nuclear Antigens |
| Comments/Corrections | |
Comment In:
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J Infect Dis. 2005 Nov 1;192(9):1503-4
[PMID:
16206063
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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