Document Detail


The proprotein convertase furin in human trophoblast: Possible role in promoting trophoblast cell migration and invasion.
MedLine Citation:
PMID:  19853298     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Furin, a proprotein convertase (PC), is ubiquitously expressed and implicated in many physiological and pathological processes. This study is aimed to identify the role of furin in human trophoblast invasion and migration. Furin was found to be highly expressed in placental villi of both rhesus monkeys and human beings during early pregnancy. Specifically, furin was found in trophoblast column and trophoblast shell, regions where highly invasive cytotrophoblast cells invade the maternal decidua during human placentation. To determine whether furin plays any role in trophoblast invasion and migration, we employed human extravillous HTR8/SVneo cells in Matrigel invasion and transwell migration assays. Knocking-down furin expression by siRNA significantly inhibited invasion and migration of HTR8/SVneo cells (P<0.01), with corresponding decrease of matrix metalloproteinase-9 (MMP-9) activities. In contrast, over-expression of furin markedly increased cell invasion and migration (P<0.01), accompanied by significant increase of MMP-9 activities. Furthermore, furin siRNA significantly increased the levels of both tissue inhibitors of MMPs (TIMP)-1 and -2. Our results suggest that furin may play an important role in the invasion and migration of human trophoblast cells during early pregnancy.
Authors:
Z Zhou; T Shen; B-H Zhang; X-Y Lv; H-Y Lin; C Zhu; L-Q Xue; H Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-10-22
Journal Detail:
Title:  Placenta     Volume:  30     ISSN:  1532-3102     ISO Abbreviation:  Placenta     Publication Date:  2009 Nov 
Date Detail:
Created Date:  2009-11-02     Completed Date:  2010-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8006349     Medline TA:  Placenta     Country:  England    
Other Details:
Languages:  eng     Pagination:  929-38     Citation Subset:  IM    
Affiliation:
Institute of Zoology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Cell Movement / physiology
Female
Furin / metabolism,  physiology*
Gene Knockdown Techniques
Humans
Macaca mulatta
Male
Matrix Metalloproteinase 9 / metabolism
Placentation / physiology*
Pregnancy
Trophoblasts / enzymology*,  physiology
Chemical
Reg. No./Substance:
EC 3.4.21.75/Furin; EC 3.4.24.35/Matrix Metalloproteinase 9

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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