| The proprotein convertase furin in human trophoblast: Possible role in promoting trophoblast cell migration and invasion. | |
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MedLine Citation:
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PMID: 19853298 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Furin, a proprotein convertase (PC), is ubiquitously expressed and implicated in many physiological and pathological processes. This study is aimed to identify the role of furin in human trophoblast invasion and migration. Furin was found to be highly expressed in placental villi of both rhesus monkeys and human beings during early pregnancy. Specifically, furin was found in trophoblast column and trophoblast shell, regions where highly invasive cytotrophoblast cells invade the maternal decidua during human placentation. To determine whether furin plays any role in trophoblast invasion and migration, we employed human extravillous HTR8/SVneo cells in Matrigel invasion and transwell migration assays. Knocking-down furin expression by siRNA significantly inhibited invasion and migration of HTR8/SVneo cells (P<0.01), with corresponding decrease of matrix metalloproteinase-9 (MMP-9) activities. In contrast, over-expression of furin markedly increased cell invasion and migration (P<0.01), accompanied by significant increase of MMP-9 activities. Furthermore, furin siRNA significantly increased the levels of both tissue inhibitors of MMPs (TIMP)-1 and -2. Our results suggest that furin may play an important role in the invasion and migration of human trophoblast cells during early pregnancy. |
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Authors:
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Z Zhou; T Shen; B-H Zhang; X-Y Lv; H-Y Lin; C Zhu; L-Q Xue; H Wang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-10-22 |
Journal Detail:
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Title: Placenta Volume: 30 ISSN: 1532-3102 ISO Abbreviation: Placenta Publication Date: 2009 Nov |
Date Detail:
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Created Date: 2009-11-02 Completed Date: 2010-01-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8006349 Medline TA: Placenta Country: England |
Other Details:
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Languages: eng Pagination: 929-38 Citation Subset: IM |
Affiliation:
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Institute of Zoology, Chinese Academy of Sciences, Chaoyang District, Beijing 100101, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Line Cell Movement / physiology Female Furin / metabolism, physiology* Gene Knockdown Techniques Humans Macaca mulatta Male Matrix Metalloproteinase 9 / metabolism Placentation / physiology* Pregnancy Trophoblasts / enzymology*, physiology |
| Chemical | |
Reg. No./Substance:
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EC 3.4.21.75/Furin; EC 3.4.24.35/Matrix Metalloproteinase 9 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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