| A prophenoloxidase from Artemia sinica: cDNA cloning, expression and activity analysis during early development. | |
| | |
MedLine Citation:
|
PMID: 21963856 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
To understand the defense mechanisms of Crustacean animals, brine shrimp Artemia sinica prophenoloxidase (AsproPO) cDNA was cloned and its expression at early developmental stages was examined by reverse-transcription PCR (RT-PCR) and semi-quantitative RT-PCR, and activity of phenoloxidase (PO) at different developmental stages was further detected by using l-3,4-dihydroxyphenylalanine (l-DOPA) as a specific substrate in this study. It was found that the full-length of AsproPO cDNA is 2125 bp and it contains an open reading frame of 2100 bp encoding a protein of 699 amino acids. The deduced amino acid sequence of AsproPO has two putative copper binding sites highly conserved in Arthropods. Semi-quantitative RT-PCR analyses showed that the gene of AsproPO expressed at Emergence, Instar I and Instar II stages but did not at 0 h and 6 h stages. Activity measurement showed that PO activity could only be detected at Instar II stage but the other measured stages. All these implied that Artemia proPO immune system was complexly modulated during early development. |
| | |
Authors:
|
Tingjun Fan; Liyan Wang; Xianyuan Fan; Bin Xu; Miaomiao Yu; Guojian Jiang |
Related Documents
:
|
21106126 - T3sedb: data warehousing of virulence effectors secreted by the bacterial type iii secr... 18671746 - Phylogenetic relationships among species of pichia, issatchenkia and williopsis determi... 18176856 - Orientobilharzia turkestanicum is grouped within african schistosomes based on phylogen... 20813986 - Molecular phylogeny of the sacoglossa, with a discussion of gain and loss of kleptoplas... 8863746 - Sequence analysis of the beta b2-crystallin cdna of hamster containing a domain conserv... 12148636 - Taxonomic dissection of the streptococcus bovis group by analysis of manganese-dependen... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-9-24 |
Journal Detail:
|
Title: Fish & shellfish immunology Volume: - ISSN: 1095-9947 ISO Abbreviation: - Publication Date: 2011 Sep |
Date Detail:
|
Created Date: 2011-10-3 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9505220 Medline TA: Fish Shellfish Immunol Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011. Published by Elsevier Ltd. |
Affiliation:
|
Department of Marine Biology, College of Marine Life Sciences, Ocean University of China, Yushan Road 5, Qingdao 266003, People's Republic of China. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Checkpoint control and cancer.
Next Document: Over-expression, purification and immune responses to Aeromonas hydrophila AL09-73 flagellar protein...