| The properties of gelatin-poly (gamma-glutamic acid) hydrogels as biological glues. | |
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MedLine Citation:
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PMID: 17502686 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The influence of the molecular weight and the type of gelatin (A or B), as well as the molecular weight of poly (gamma-glutamic acid) (gamma-PGA), on the properties of gelatin/gamma-PGA mixed bioadhesives were studied. The gelation of the system was enhanced by a crosslinker, 1-(3-dimethylaminopropyl)-3-(ethylcarbodiimide) hydrochloride (EDC). The gelation time of the bioadhesives was analyzed using rheological measurements. The results indicated that the type of gelatin was a critical factor in determining the gelation time of the biological glues. The mixed glues had greater bonding strength and smaller gelation times as the molecular weight of gamma-PGA or gelatin increased. The swelling ratio decreased and the denaturation temperature increased upon raising the EDC concentration, indicating a greater degree of crosslinking at higher EDC concentrations. The mixed glues crosslinked with various concentrations of EDC (1.7-2.5%) showed no cytotoxicity to fibroblasts. In addition, no significant inflammatory response was observed in the rat subcutaneous implantation. The bioadhesives based on gelatin/gamma-PGA remained at the site for 7 days while the fibrin glue had almost completely degraded. By choosing the appropriate gelatin type and higher molecular weight gamma-PGA in the mixtures, the gelatin/gamma-PGA biological glues could serve as soft tissue adhesives. Rheological characterization was essential in the evaluation of biological glues. |
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Authors:
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Shan-Hui Hsu; Chen-Huan Lin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biorheology Volume: 44 ISSN: 0006-355X ISO Abbreviation: Biorheology Publication Date: 2007 |
Date Detail:
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Created Date: 2007-05-15 Completed Date: 2007-06-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372526 Medline TA: Biorheology Country: Netherlands |
Other Details:
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Languages: eng Pagination: 17-28 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan, ROC. shhsu@nchu.edu.tw |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Death / drug effects Cellulitis / chemically induced, pathology Dose-Response Relationship, Drug Drug Combinations Fibroblasts / drug effects Gelatin / chemistry*, therapeutic use, toxicity Hydrogels / chemistry, toxicity Molecular Weight Polyglutamic Acid / chemistry*, therapeutic use Rats Rats, Sprague-Dawley Rheology Shear Strength Swine Tissue Adhesives / chemistry*, toxicity |
| Chemical | |
Reg. No./Substance:
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0/Drug Combinations; 0/Hydrogels; 0/Tissue Adhesives; 0/gelatin-polyglutamic acid glue; 25513-46-6/Polyglutamic Acid; 9000-70-8/Gelatin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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