Document Detail


A promising broad spectrum antimicrobial red fluorescent protein present in silkworm excreta.
MedLine Citation:
PMID:  20606304     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purified silkworm excretory red fluorescent protein (SE-RFP) has exhibited a potent broad spectrum antimicrobial activity. The anti-microbial assays of purified SE-RFP against several pathogenic bacterial (both Gram positive and Gram negative) and fungal strains were performed by agar cup plate method. The minimum inhibitory concentration (MIC) of SE-RFP against pathogenic bacteria and fungi was evaluated by agar dilution technique. The SE-RFP has exhibited highest activity (lowest minimum inhibitory concentration and largest zone of inhibition) against Staphylococcus aureus and Candida albicans among the tested bacteria and fungi, respectively. For the first time, we are reporting here the bioactivity of a red fluorescent protein purified from the silkworm excreta against clinically important bacteria and fungi. The bioactive SE-RFP has two absorption peaks at 280 and 603 nm and, it has exhibited fluorescence emission peaks at 334 and 619 nm upon exciting at 280 and 603 nm, respectively. The SE-RFP being an aqua-soluble, economically feasible and eco-friendly protein, it can therefore be used for the practical applications as an effective antimicrobial agent.
Authors:
Kalyankumar Marikallappa Matti; Chandrashekhar Jagadeeshshastri Savanurmath; Shivayogeppa Bangareppa Hinchigeri
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  33     ISSN:  1347-5215     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2010  
Date Detail:
Created Date:  2010-07-07     Completed Date:  2010-11-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  1143-7     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Karnatak University, Dharwad-580003, India.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anti-Infective Agents / pharmacology*
Bombyx / chemistry*
Feces / chemistry*
Fungi / drug effects
Gram-Negative Bacteria / drug effects
Gram-Positive Bacteria / drug effects
Luminescent Proteins / pharmacology*
Microbial Sensitivity Tests
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Chemical
Reg. No./Substance:
0/Anti-Infective Agents; 0/Luminescent Proteins; 0/red fluorescent protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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