| The promise and peril of continuous in vitro evolution. | |
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MedLine Citation:
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PMID: 15999246 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Experimental evolution methods can be used to address and illuminate issues central to the understanding of evolutionary theory. One of the most powerful of these methods involves the in vitro evolution of nucleic acid enzymes, taking advantage of the direct relationship between the genotype of a nucleic acid sequence and the phenotype of its associated catalytic function. This review and commentary focuses on the past, present, and future potential of systems for the continuous in vitro evolution of nucleic acid enzymes as tools for modeling evolutionary processes in biology. It offers a candid appraisal of both the strengths and the limitations of these systems. |
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Authors:
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Glenn C Johns; Gerald F Joyce |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Review Date: 2005-06-27 |
Journal Detail:
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Title: Journal of molecular evolution Volume: 61 ISSN: 0022-2844 ISO Abbreviation: J. Mol. Evol. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-09-14 Completed Date: 2006-01-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0360051 Medline TA: J Mol Evol Country: Germany |
Other Details:
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Languages: eng Pagination: 253-63 Citation Subset: IM |
Affiliation:
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Departments of Chemistry and Molecular Biology and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA. gjoyce@scripps.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Directed Molecular Evolution
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methods*,
standards,
trends Ligases / genetics, metabolism Nucleic Acid Conformation RNA, Catalytic / chemistry, classification, genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/RNA, Catalytic; EC 6.-/Ligases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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