| A prolyl oligopeptidase inhibitor, Z-Pro-Prolinal, inhibits glyceraldehyde-3-phosphate dehydrogenase translocation and production of reactive oxygen species in CV1-P cells exposed to 6-hydroxydopamine. | |
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MedLine Citation:
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PMID: 16942854 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We studied the ability of prolyl oligopeptidase (POP) inhibitors, Z-Pro-Prolinal and JTP-4819, to prevent translocation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and formation of reactive oxygen species (ROS), in 6-hydroxydopamine (6-OHDA) and cytosine arabinoside (Ara-C) treated monkey fibroblast (CV1-P) and human neuroblastoma (SH-SY5Y) cells. The cells were pretreated with POP inhibitors (30 min) before addition of toxicants. GAPDH was analyzed by Western hybridization, ROS by fluorescent 2'7'-dichlorodihydro-fluorescein diacetate, and viability by the MTT method. Both toxicants induced GAPDH translocation to the particulate fraction (mitochondria and nuclei). Z-Pro-Prolinal was able to inhibit the translocation in 6-OHDA-exposed CV1-P cells. In SH-SY5Y cells and in JTP-4819 pretreated cells, no prevention of translocation was seen. However, the intensity of GAPDH in cytosolic fraction increased. Both inhibitors blocked 6-OHDA-induced ROS-production to the control level in CV1-P but, not in SH-SY5Y cells, without affecting their viability. In conclusion, POP inhibitors are able to prevent certain cell stress related factors such as ROS production or GAPDH translocation. |
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Authors:
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Katja A Puttonen; Sárka Lehtonen; Atso Raasmaja; Pekka T Männistö |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-07-14 |
Journal Detail:
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Title: Toxicology in vitro : an international journal published in association with BIBRA Volume: 20 ISSN: 0887-2333 ISO Abbreviation: Toxicol In Vitro Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-10-18 Completed Date: 2006-12-26 Revised Date: 2009-04-10 |
Medline Journal Info:
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Nlm Unique ID: 8712158 Medline TA: Toxicol In Vitro Country: England |
Other Details:
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Languages: eng Pagination: 1446-54 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Toxicology, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland. katja.puttonen@uku.fi |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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biosynthesis,
genetics Blotting, Western Cell Line Cell Survival / drug effects Cytarabine / antagonists & inhibitors, toxicity Dipeptides / pharmacology* Glyceraldehyde-3-Phosphate Dehydrogenases / metabolism* Humans Oxidopamine / antagonists & inhibitors*, toxicity* Protease Inhibitors / pharmacology* Protein Transport / drug effects Proto-Oncogene Proteins c-bcl-2 / metabolism Pyrrolidines / pharmacology Reactive Oxygen Species / metabolism* Serine Endopeptidases / metabolism* Tetrazolium Salts Thiazoles Tumor Suppressor Protein p53 / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Dipeptides; 0/JTP 4819; 0/Protease Inhibitors; 0/Proto-Oncogene Proteins c-bcl-2; 0/Pyrrolidines; 0/Reactive Oxygen Species; 0/Tetrazolium Salts; 0/Thiazoles; 0/Tumor Suppressor Protein p53; 1199-18-4/Oxidopamine; 147-94-4/Cytarabine; 298-93-1/thiazolyl blue; 86925-97-5/N-benzyloxycarbonylprolylprolinal; EC 1.2.1.-/Glyceraldehyde-3-Phosphate Dehydrogenases; EC 3.4.21.-/Serine Endopeptidases; EC 3.4.21.26/prolyl oligopeptidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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