Document Detail

The proliferative state of granulocytic progenitor cells in human blood and marrow.
MedLine Citation:
PMID:  6966187     Owner:  NLM     Status:  MEDLINE    
A double layer agar technique was used to investigate the proliferative state of granulocytic progenitor cells (Colony Forming Units in Culture; CFUc) in human peripheral blood and bone marrow. The sensitivity of the progenitor cells to the S-phase specific agent, hydroxyurea, was used as an index of the proportion of cells engaged in DNA synthesis. In the presence of low concentrations of colony stimulating factor (CSF) the CFUc were found to be virtually insensitive to the drug. However, when cultured in the presence of increasing concentrations of CSF the proportion of CFUc apparently killed by hydroxyurea increased to a maximum of 23% for those cells in the blood and 39% for those in the marrow. The results indicate that CFUc which are slowly proliferating are sensitive to low concentrations of CSF. In contrast, those CFUc which are proliferating more rapidly require high concentrations of CSF before they will form colonies in culture. A model has been devised which suggests that as CFUc mature, their cell cycle time shortens and their sensitivity to CSF decreases.
G Standen; M A Philip; J Fletcher
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell and tissue kinetics     Volume:  13     ISSN:  0008-8730     ISO Abbreviation:  Cell Tissue Kinet     Publication Date:  1980 Jan 
Date Detail:
Created Date:  1980-07-22     Completed Date:  1980-07-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0174107     Medline TA:  Cell Tissue Kinet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  105-13     Citation Subset:  IM    
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MeSH Terms
Blood Cells / cytology*
Cell Cycle
Colony-Stimulating Factors / pharmacology
Granulocytes / cytology*
Hematopoietic Stem Cells / cytology*,  drug effects
Hydroxyurea / pharmacology
Reg. No./Substance:
0/Colony-Stimulating Factors; 127-07-1/Hydroxyurea

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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