Document Detail

The proliferative effects of ghrelin on human gastric cancer AGS cells.
MedLine Citation:
PMID:  22908970     Owner:  NLM     Status:  In-Data-Review    
OBJECTIVE:   To investigate the role of ghrelin in the gastric cancer cell line AGS and its probable mechanism.
METHODS:   Cell proliferation was detected by MTT assay after treated with ghrelin or des-acyl ghrelin. The expression of growth hormone secretagogue receptor 1a (GHS-R1a) and 1b (GHS-R1b) mRNA was detected using reverse transcription polymerase chain reaction (RT-PCR). The activity of extracellular signal-regulated kinase 1/2 (ERK1/2) and Akt was measured by Western blot in cells either treated with ghrelin or inhibitors for ERK1/2 and phosphoinositide-3 kinase (PI3K). The distribution of cell cycle phases was determined by flow cytometry analysis of DNA content.
RESULTS:   GHS-R1a and GHS-R1b mRNA were expressed in the AGS cells. Ghrelin and des-acyl ghrelin induced AGS cell proliferation at concentrations of 1 nmol/L and 10 nmol/L but had no proliferative effect at a concentration of 100 nmol/L. The treatment of AGS cells with 10 nmol/L of ghrelin and des-acyl ghrelin resulted in the progression of the increased cells in the S phase. ERK1/2 and Akt were activated by ghrelin and des-acyl ghrelin. Specific ERK1/2 inhibitor PD98059 and PI3K inhibitor wortmannin reduced phosphorylation of ERK1/2 and Akt, respectively and blocked ghrelin- and des-acyl ghrelin-induced AGS cell proliferation.
CONCLUSION:   Ghrelin and des-acyl ghrelin stimulate the proliferation of gastric cancer cells via the activation of the ERK1/2 and PI3K/Akt pathway.
Pei Ying Tian; Xiao Ming Fan
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of digestive diseases     Volume:  13     ISSN:  1751-2980     ISO Abbreviation:  J Dig Dis     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-08-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101302699     Medline TA:  J Dig Dis     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  453-8     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors. Journal of Digestive Diseases © 2012 Chinese Medical Association Shanghai Branch, Chinese Society of Gastroenterology, Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine and Wiley Publishing Asia Pty Ltd.
Department of Gastroenterology, Jinshan Hospital, Fudan UniversityDepartment of Internal Medicine, Shanghai Medical College of Fudan University, Shanghai, China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Nutritional support in acute pancreatitis.
Next Document:  Association between inflammatory bowel disease gene 5 (IBD5) and interleukin-23 receptor (IL23R) gen...