Document Detail

The proliferation of spermatogonia in normal and pathological human seminiferous epithelium: an immunohistochemical study using monoclonal antibodies against Ki-67 protein and proliferating cell nuclear antigen.
MedLine Citation:
PMID:  9570268     Owner:  NLM     Status:  MEDLINE    
The quantitative distribution pattern of Ki-67 protein and proliferating cell nuclear antigen (PCNA) immunoreactivity was studied in human testis biopsies. In normal seminiferous epithelium Ki-67 is expressed in nuclei of spermatogonia, while PCNA additionally occurs in nuclei of primary spermatocytes. The staining pattern of spermatogonia is as follows (Ki-67-positive/PCNA-positive): 26.6 +/- 12.4%/46.3 +/- 9.5%. No stage-dependent differences were found. Biopsies with mixed atrophy (score < or =7) showed a significant (P < 0.05) decrease of immunopositive spermatogonia to 19.9 +/- 3.0%/31.4 +/- 5.7% (score 1) with minimal variation between different samples (score 7 to 1). Associated with defined histological defects such as hypospermatogenesis (hyp), spermatogenic arrest at the level of spermatids (sda), spermatocytes (sca) or spermatogonia (sga), however, there was a significant (P < 0.05) decrease of Ki-67 staining in tubules showing hyp (28.6 +/- 8.8%), sda (25.6 +/- 9.3%), sca (23.7 +/- 9.3%) and sga (16.2 +/- 6.0%) and of PCNA staining in sca (32.2 +/- 11.8%) and sga (20.0 +/- 9.5%), respectively. The decrease of immunoreactive spermatogonia did not correspond to elevation of follicle stimulating hormone (FSH). These data demonstrate that the low spermatogenic efficiency in infertile men is not only due to postmeiotic events, but also to a decrease in the meiotic activity of spermatogonia, and is not related to serum FSH.
K Steger; I Aleithe; H Behre; M Bergmann
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular human reproduction     Volume:  4     ISSN:  1360-9947     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-06-08     Completed Date:  1998-06-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  227-33     Citation Subset:  IM    
Institute of Anatomy and Cell Biology, University of Halle, Halle (Saale), Germany.
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MeSH Terms
Antibodies, Monoclonal
Cell Division
Cell Nucleus / chemistry
Follicle Stimulating Hormone / analysis
Ki-67 Antigen / analysis*
Oligospermia / pathology
Proliferating Cell Nuclear Antigen / analysis*
Seminiferous Epithelium / cytology*,  pathology*
Spermatogenesis / physiology
Spermatogonia / chemistry,  cytology*,  pathology
Testis / chemistry
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Ki-67 Antigen; 0/Proliferating Cell Nuclear Antigen; 9002-68-0/Follicle Stimulating Hormone

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