Document Detail


A prognostic signature of defective p53-dependent G1 checkpoint function in melanoma cell lines.
MedLine Citation:
PMID:  22540896     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Melanoma cell lines and normal human melanocytes (NHM) were assayed for p53-dependent G1 checkpoint response to ionizing radiation (IR)-induced DNA damage. Sixty-six percent of melanoma cell lines displayed a defective G1 checkpoint. Checkpoint function was correlated with sensitivity to IR with checkpoint-defective lines being radio-resistant. Microarray analysis identified 316 probes whose expression was correlated with G1 checkpoint function in melanoma lines (P≤0.007) including p53 transactivation targets CDKN1A, DDB2, and RRM2B. The 316 probe list predicted G1 checkpoint function of the melanoma lines with 86% accuracy using a binary analysis and 91% accuracy using a continuous analysis. When applied to microarray data from primary melanomas, the 316 probe list was prognostic of 4-yr distant metastasis-free survival. Thus, p53 function, radio-sensitivity, and metastatic spread may be estimated in melanomas from a signature of gene expression.
Authors:
Craig Carson; Bernard Omolo; Haitao Chu; Yingchun Zhou; Maria J Sambade; Eldon C Peters; Patrick Tompkins; Dennis A Simpson; Nancy E Thomas; Cheng Fan; Alain Sarasin; Philippe Dessen; Janiel M Shields; Joseph G Ibrahim; William K Kaufmann
Related Documents :
21528296 - Dexamethasone apoptosis induction and glucocorticoid receptor levels in cultured normal...
21481226 - Oncostatin m promotes stat3 activation, vegf production, and invasion in osteosarcoma c...
25268196 - Clear cell melanoma: a cutaneous clear cell malignancy.
21195136 - Immortalization and functional characterization of rat arachnoid cell lines.
21455586 - Ursodeoxycholic acid-induced inhibition of dlc1 protein degradation leads to suppressio...
21575656 - Fabrication of chitin-chitosan/nano zro(2) composite scaffolds for tissue engineering a...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2012-06-01
Journal Detail:
Title:  Pigment cell & melanoma research     Volume:  25     ISSN:  1755-148X     ISO Abbreviation:  Pigment Cell Melanoma Res     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-06-20     Completed Date:  2012-10-17     Revised Date:  2014-04-14    
Medline Journal Info:
Nlm Unique ID:  101318927     Medline TA:  Pigment Cell Melanoma Res     Country:  England    
Other Details:
Languages:  eng     Pagination:  514-26     Citation Subset:  IM    
Copyright Information:
© 2012 John Wiley & Sons A/S.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Cell Line, Tumor
Cyclin-Dependent Kinase Inhibitor p16 / genetics,  metabolism
Cyclin-Dependent Kinase Inhibitor p21 / genetics,  metabolism
DNA Probes / metabolism
G1 Phase Cell Cycle Checkpoints / genetics*
Gene Expression Profiling*
Gene Expression Regulation, Neoplastic
Humans
Melanocytes / metabolism,  pathology
Melanoma / diagnosis,  genetics*,  pathology
Prognosis
Skin Neoplasms / diagnosis,  genetics*,  pathology
Tumor Suppressor Protein p53 / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
CA106991/CA/NCI NIH HHS; CA16086/CA/NCI NIH HHS; ES014635/ES/NIEHS NIH HHS; ES07126/ES/NIEHS NIH HHS; ES10126/ES/NIEHS NIH HHS; P01 ES014635/ES/NIEHS NIH HHS; P30 CA016086/CA/NCI NIH HHS; P30 ES010126/ES/NIEHS NIH HHS; R01 GM070335/GM/NIGMS NIH HHS; T32 CA106209/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Cyclin-Dependent Kinase Inhibitor p16; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/DNA Probes; 0/Tumor Suppressor Protein p53
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The absence of voiding symptoms in men with a prostate-specific antigen (PSA) concentration of ?3.0?...
Next Document:  Does doctors' workload impact supervision and ward activities of final-year students? A prospective ...