Document Detail


A progestin and an estrogen regulate early stages of oogenesis in fish.
MedLine Citation:
PMID:  17687117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Using two species of teleost fish, Japanese huchen (Hucho perryi) and common carp (Cyprinus carpio), we investigated whether sex steroids are involved in early oogenesis in vitro. Ovarian fragments were cultured to examine the effects of a progestin, 17alpha, 20beta-dihydroxy-4-pregnen-3-one (DHP), and an estrogen, estradiol-17 beta (E2). DHP and E2 significantly promoted DNA synthesis in ovarian germ cells, as judged by 5-bromo-2-deoxyuridine (BrdU) incorporation into these cells. Furthermore, to detect the initiation of the first meiotic division of early oogenesis, we assessed ultrastructurally the occurrence of synaptonemal complexes (SCs) and analyzed by immunohistochemistry the expression of a meiosis-specific marker, Spo11. In huchen, a higher percentage of oocytes with SC was seen in DHP-treated ovarian fragments than in control or E2-treated ovarian fragments. Spo11 was expressed in germ cells after DHP treatment of carp ovarian explants. These data suggest that the progression of germ cells through early oogenesis involves two sex steroids: E2, which acts directly on oogonial proliferation, and DHP, which acts directly on the initiation of the first meiotic division of oogenesis.
Authors:
Chiemi Miura; Toshitsugu Higashino; Takeshi Miura
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-08-08
Journal Detail:
Title:  Biology of reproduction     Volume:  77     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-22     Completed Date:  2008-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  822-8     Citation Subset:  IM    
Affiliation:
Laboratory of Fish Reproductive Physiology, Faculty of Agriculture, Ehime University, Ehime 790-8566, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bromodeoxyuridine / metabolism
Carps / physiology*
DNA Replication / drug effects
Estradiol / blood,  pharmacology,  physiology*
Estrogens / pharmacology,  physiology
Female
Hydroxyprogesterones / blood,  metabolism*,  pharmacology
Oogenesis*
Oogonia / drug effects,  physiology
Ovary / cytology,  drug effects,  physiology*
Progestins / pharmacology,  physiology
Salmonidae / physiology*
Chemical
Reg. No./Substance:
0/Estrogens; 0/Hydroxyprogesterones; 0/Progestins; 10456-50-5/17,20-dihydroxy-4-pregnen-3-one; 50-28-2/Estradiol; 59-14-3/Bromodeoxyuridine

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