| Excretory/secretory products from plerocercoids of Spirometra erinaceieuropaei suppress the TNF-alpha gene expression by reducing phosphorylation of ERK1/2 and p38 MAPK in macrophages. | |
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MedLine Citation:
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PMID: 12117498 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We previously reported that excretory/secretory products from plerocercoids of Spirometra erinaceieuropaei suppress gene expression and production of tumour necrosis factor-alpha in murine macrophages stimulated with lipopolysaccharide. The present study investigated the suppressive mechanisms of tumour necrosis factor-alpha mRNA by excretory/secretory products in lipopolysaccharide-stimulated murine macrophages. Electrophoretic mobility shift assay and supershift assay revealed that neither nuclear translocation of nuclear factor-kappa B nor conformation of the p50/p65 nuclear factor-kappa B subunits was affected by the treatment of excretory/secretory products in lipopolysaccharide-stimulated macrophages. Inhibition of extracellular signal-regulated protein kinase 1/2 with PD98059 or p38 mitogen-activated protein kinase with SB203580 partially reduced tumour necrosis factor-alpha mRNA expression, and a combination of the two inhibitors additionally suppressed the level of tumour necrosis factor-alpha mRNA, revealing that both pathways are crucial for full induction of the gene. Northern blot analysis showed that excretory/secretory products additionally suppressed tumour necrosis factor-alpha mRNA expression in cells treated with PD98059 or SB208530 and, in turn, we found that excretory/secretory products reduced phosphorylation of extracellular signal-regulated protein kinase 1/2 and p38 mitogen-activated protein kinase in lipopolysaccharide-stimulated macrophages by Western blot analysis. This is the first report demonstrating that excretory/secretory products from parasites suppress tumour necrosis factor-alpha mRNA expression by reducing phosphorylation of extracellular signal-regulated protein kinase 1/2 and p38 mitogen-activated protein kinase without any effect on nuclear factor-kappa B activity in macrophages stimulated with lipopolysaccharide. We hypothesise that excretory/secretory products may enable this parasite to survive within the host. |
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Authors:
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Paramasari Dirgahayu; Soji Fukumoto; Kazutoyo Miura; Kazumitsu Hirai |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal for parasitology Volume: 32 ISSN: 0020-7519 ISO Abbreviation: Int. J. Parasitol. Publication Date: 2002 Aug |
Date Detail:
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Created Date: 2002-07-15 Completed Date: 2002-11-14 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0314024 Medline TA: Int J Parasitol Country: England |
Other Details:
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Languages: eng Pagination: 1155-62 Citation Subset: IM |
Affiliation:
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Department of Molecular Medical Zoology, Faculty of Medicine, Tottori University, 86 Nishi-cho, 683-8503 Yonago, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Factors / pharmacology* Blotting, Northern Gene Expression Regulation / drug effects* Lipopolysaccharides / pharmacology Macrophage Activation / drug effects Macrophages / drug effects*, enzymology, metabolism* Mitogen-Activated Protein Kinases / genetics, metabolism* Phosphorylation / drug effects RNA Processing, Post-Transcriptional RNA, Messenger / genetics, metabolism Spirometra / metabolism*, pathogenicity Tumor Necrosis Factor-alpha / biosynthesis, genetics* p38 Mitogen-Activated Protein Kinases |
| Chemical | |
Reg. No./Substance:
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0/Biological Factors; 0/Lipopolysaccharides; 0/RNA, Messenger; 0/Tumor Necrosis Factor-alpha; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 2.7.11.24/p38 Mitogen-Activated Protein Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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