Document Detail

In vitro production of small ruminant embryos: Late improvements and further research.
MedLine Citation:
PMID:  24650929     Owner:  NLM     Status:  Publisher    
Beyond the potential use of in vitro production of embryos (IVP) in breeding schemes, embryos are also required for the establishment of new biotechnologies such as cloning and transgenesis. Additionally, the knowledge of oocyte and embryo physiology acquired through IVP techniques may stimulate the further development of other techniques such as marker assisted and genomic selection of preimplantation embryos, and also benefit assisted procreation in human beings. Efficient in vitro embryo production is currently a major objective for livestock industries, including small ruminants. The heterogeneity of oocytes collected from growing follicles by laparoscopic ovum pick up or in ovaries of slaughtered females, remains an enormous challenge for IVM success, and still limits the rate of embryo development. In addition, the lower quality of the IVP embryos, compared with their in vivo-derived counterparts, translates into poor cryosurvival, which restricts the wider use of this promising technology. Therefore, many studies have been reported in an attempt to determine the most suitable conditions for IVM, IVF, and in vitro development to maximize embryo production rate and quality. This review aims to present the current panorama of IVP production in small ruminants, describing important steps for its success, reporting the recent advances and also the main obstacles identified for its improvement and dissemination.
Joanna Maria Gonçalves de Souza-Fabjan; Barbara Panneau; Nicolas Duffard; Yann Locatelli; José Ricardo de Figueiredo; Vicente José de Figueirêdo Freitas; Pascal Mermillod
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Publication Detail:
Type:  REVIEW     Date:  2014-2-8
Journal Detail:
Title:  Theriogenology     Volume:  -     ISSN:  1879-3231     ISO Abbreviation:  Theriogenology     Publication Date:  2014 Feb 
Date Detail:
Created Date:  2014-3-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421510     Medline TA:  Theriogenology     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014 Elsevier Inc. All rights reserved.
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