Document Detail


The production of reactive oxygen species, gene expression, and enzymatic activity in quails subjected to acute heat stress.
MedLine Citation:
PMID:  23148249     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The aim of the present study was to evaluate the affect of acute heat stress on the production of mitochondrial reactive oxygen species (ROS), the gene expression of the avian uncoupling protein (avUCP) and glutathione peroxidase (GPX 7), and the activity of the enzyme glutathione peroxidase in the liver of meat quails. Two groups of 15 meat quails (Coturnix coturnix japonica) that were 23 days of age were initially housed individually in metallic cages. A period of seven days was provided for the two birds groups to adapt to the cages and to a thermoneutral environment at 25°C with 60% relative humidity. At 30 day of age, 15 quails were exposed to 34°C for 24 hours, humidity 60%- heat stress (HS) treatment, whereas control quails (n=15) were kept at 25°C. To analyze the production of ROS, four quails from each treatment group were slaughtered, and their livers were collected for mitochondrial isolation and to measure the subsequent production of ROS by the mitochondria. Additionally, the livers of six animals from each treatment group were collected for total RNA extraction. The cDNA was amplified using primers specific for the target genes, and expression was analyzed using the real-time polymerase reaction (qRT-PCR). Five animals from each treatment group were slaughtered to analyze glutathione peroxidase (GSH-Px) activity, which was determined by using of hydrogen peroxide (H(2)O(2)), and based on measuring the amount NADPH oxidized. A greater amount of mitochondrial ROS was found in HS animals (0.34 vs. 0.22 nm of ROS produced/minute/milligram of protein, P<0.05) for the reactions that contained only rotenone and in the reactions that were performed with rotenone and antimycin (0.31 vs. 0.23 nm of ROS produced/minute/milligram of protein, P<0.05). Concomitantly, the birds that were subjected to acute heat stress and had a greater amount of ROS production expressed less avUCP mRNA (0.75 UA vs. 0.87 UA, P<0.05) and more GPX 7 mRNA (2.37 UA vs. 1.17 UA, P<0.01). The HS quails displayed significantly higher glutathione peroxidase activity in their hepatocytes (47.8 vs. 39.6 nmol of NADPH oxidized per mg of protein per minute, P<0.05). Thus, acute heat stress at 34°C for 24 hours affects the production of mitochondrial ROS, the expression of avUCP and GPX 7 mRNA, and the activity of the glutathione peroxidase enzyme in the liver of meat quails.
Authors:
A P Del Vesco; E Gasparino
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-12
Journal Detail:
Title:  Journal of animal science     Volume:  -     ISSN:  1525-3163     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Animal Science of the State University of Maringá - Maringá, Paraná - Brazil. Postal code: 87020-900.
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