Document Detail

The production and characterisation of trichotoxin peptaibols, by Trichoderma asperellum.
MedLine Citation:
PMID:  18816522     Owner:  NLM     Status:  MEDLINE    
Trichoderma spp. are regularly found as a constituent of the mycoflora of many soils and are noted for their antagonistic activity against bacteria and other fungi. This latter property is the basis for the widespread interest in their use in the biological control of soil-borne fungal plant pathogens. This antagonism is partly based on their ability to produce an impressive inventory of secondary metabolites. An important group of bioactive metabolites produced by Trichoderma spp. are the non-ribosomal peptides (NRPs), especially the peptaibols. A virulent antagonistic strain, T. asperellum, which had been used in biological control strategies in Malaysia and previously examined for mycolytic enzyme production, has been studied for its potential for peptaibol production. The present research demonstrated the ability of T. asperellum to produce at least two metabolites which were identified as acid trichotoxin 1704E (Ac-Aib-Gly-Aib-Leu-Aib-Gln-Aib-Aib-Aib-Ala-Ala-Aib-Pro-Leu-Aib-Iva-Glu-Vol) and neutral trichotoxin 1717A (Ac-Aib-Gly-Aib-Leu-Aib-Gln-Aib-Aib-Aib-Ala-Aib-Aib-Pro-Leu-Aib-Iva-Gln-Vol). Addition of free Aib to the culture medium enhanced the production of trichotoxins. Biological activity of these substances was investigated against Bacillus stearothermophilus. The general characteristics of peptaibols, also found in the trichotoxins, include the presence of high proportions of the uncommon amino acid Aib, the protection of the N- and C-termini by an acetyl group and reduction of the C-terminus to 2-amino alcohols, respectively, amphipathy and microheterogeneity.
Chanikul Chutrakul; Marcos Alcocer; Kevin Bailey; John F Peberdy
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Chemistry & biodiversity     Volume:  5     ISSN:  1612-1880     ISO Abbreviation:  Chem. Biodivers.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-10-01     Completed Date:  2008-11-18     Revised Date:  2008-11-26    
Medline Journal Info:
Nlm Unique ID:  101197449     Medline TA:  Chem Biodivers     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  1694-706     Citation Subset:  IM    
Microbiology Group, School of Biology, University of Nottingham, University Park, Nottingham NG72RD, UK.
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MeSH Terms
Anti-Bacterial Agents / biosynthesis,  chemistry,  isolation & purification,  pharmacology
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Mass Spectrometry
Microbial Viability / drug effects
Peptaibols / biosynthesis*,  chemistry*,  isolation & purification,  pharmacology
Peptides / chemistry*,  metabolism*
Trichoderma / chemistry*,  metabolism*
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Peptaibols; 0/Peptides; 65454-19-5/trichotoxin
Erratum In:
Chem Biodivers. 2008 Nov;5(11):2464

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