Document Detail


The production of alamethicins by Trichoderma spp.
MedLine Citation:
PMID:  3664367     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The production of polypeptides containing a high percentage of 2-methylalanine residues by a number of isolates of Trichoderma spp. has been examined. It has been shown that good yields (0.5-1.0 g L-1) can be achieved on synthetic media provided an insoluble carbohydrate is included and provided single-spore isolates that have this production ability are selected from time to time. Such yields could not be obtained on any single nitrogen source investigated, but a mixture of potassium nitrate, glutamine, and 2-methylalanine was effective. It was shown that at least eight polypeptides were produced in shake-flask or tank fermentation and that the proportions of these metabolites depended on the fermentation temperature, its pH, age, and aeration. Fermentation conditions for enhancing the production (independently) of two of the metabolites at the expense of the others are given. These two metabolites have been obtained in crystalline form and details of some of their physical and chemical properties are given.
Authors:
D Brewer; F G Mason; A Taylor
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Canadian journal of microbiology     Volume:  33     ISSN:  0008-4166     ISO Abbreviation:  Can. J. Microbiol.     Publication Date:  1987 Jul 
Date Detail:
Created Date:  1987-12-01     Completed Date:  1987-12-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0372707     Medline TA:  Can J Microbiol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  619-25     Citation Subset:  IM    
Affiliation:
National Research Council of Canada, Atlantic Research Laboratory, Halifax, N.S.
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MeSH Terms
Descriptor/Qualifier:
Alamethicin / analysis,  biosynthesis*,  isolation & purification
Anti-Bacterial Agents / biosynthesis*
Chemical Phenomena
Chemistry
Chromatography, High Pressure Liquid
Culture Media
Fermentation
Hydrogen-Ion Concentration
Mitosporic Fungi / metabolism*
Nitrogen / metabolism
Temperature
Trichoderma / metabolism*
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; 0/Culture Media; 27061-78-5/Alamethicin; 7727-37-9/Nitrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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