| The product of human cytomegalovirus UL73 is a new polymorphic structural glycoprotein (gpUL73). | |
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MedLine Citation:
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PMID: 11213930 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: This work focuses on human cytomegalovirus (HCMV) UL73, which encodes for a putative transmembrane glycoprotein that is highly conserved among herpesviruses. STUDY DESIGN: pUL73 expression was analyzed both in transiently transfected and in HCMV-infected cells using a pUL73-specific antiserum by immunoblot and immunofluorescence. Sequencing analysis from several clinical isolates and laboratory-adapted strains was also performed. RESULTS: pUL73 expressed in transiently transfected cells consists in a polypeptide of the expected size (15-18 kd) with cytoplasmic localization. In infected cells, pUL73 is expressed with true-late kinetics and localizes both in perinuclear granular formations and on the cell surface. A broad band (39-53 kd), sensitive to O-glycosidase digestion was detected in purified virus. In addition, sequence analysis showed that the N-terminal portion of pUL73 from clinical isolates is highly polymorphic. CONCLUSIONS: UL73 encodes for a new structural glycoprotein (gpUL73) expressed on the cell surface of infected cells and highly polymorphic among clinical isolates. |
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Authors:
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P Dal Monte; S Pignatelli; M Mach; M P Landini |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of human virology Volume: 4 ISSN: 1090-9508 ISO Abbreviation: J. Hum. Virol. Publication Date: 2001 Jan-Feb |
Date Detail:
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Created Date: 2001-02-13 Completed Date: 2001-05-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9805755 Medline TA: J Hum Virol Country: United States |
Other Details:
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Languages: eng Pagination: 26-34 Citation Subset: IM |
Affiliation:
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Department of Clinical and Experimental Medicine, University of Bologna, Italy. dalmonte@med.unibo.it |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/AF224676; AF224677; AF224678; AF224679; AF224680; AF224681; AF224682; AF224683; AF224684; AF224685; AF224686; AF224687; AF224688 |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Base Sequence COS Cells Cercopithecus aethiops Cytomegalovirus / genetics*, isolation & purification, metabolism Cytomegalovirus Infections / virology DNA, Viral Gene Expression Glycoproteins / classification, genetics*, metabolism Humans Immunoblotting / methods Molecular Sequence Data Open Reading Frames Peptides / genetics Polymorphism, Genetic* Rabbits Recombinant Fusion Proteins / genetics Sequence Homology, Amino Acid Subcellular Fractions Viral Envelope Proteins / genetics*, metabolism Viral Structural Proteins / classification, genetics*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/Glycoproteins; 0/Peptides; 0/Recombinant Fusion Proteins; 0/Viral Envelope Proteins; 0/Viral Structural Proteins; 0/glycoprotein N, Human cytomegalovirus; 98849-88-8/FLAG peptide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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