| A procedure for small-volume brain grafting; vasopressin cells in neonatal and adult Brattleboro rats. | |
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MedLine Citation:
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PMID: 6381894 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple and reliable technique is described for the transplantation of fetal vasopressin (VP) neurons in the third ventricle of the brain of homozygous Brattleboro neonates. Small-volume grafting is introduced by microdissection of paraventricular and supraoptic areas and by pelleting the minced tissue for insertion into the transplantation cannula. Morphological and immunocytochemical evaluation yielded results in both neonatal and adult host brain that were similar to those described for anterior hypothalamic grafts in adult Brattleboro brain. The present protocol circumvents some of the general problems encountered when the use of small grafts is imperative, and is also applicable to the implantation of pelleted cell suspensions. |
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Authors:
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G J Boer; N Schluter; D M Gash |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of neuroscience methods Volume: 11 ISSN: 0165-0270 ISO Abbreviation: J. Neurosci. Methods Publication Date: 1984 May |
Date Detail:
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Created Date: 1984-10-11 Completed Date: 1984-10-11 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7905558 Medline TA: J Neurosci Methods Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 39-45 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cerebral Ventricles / analysis* Female Graft Survival Immunoenzyme Techniques Nerve Fibers / ultrastructure Nerve Regeneration* Neurons / ultrastructure Neurophysins / metabolism Paraventricular Hypothalamic Nucleus / transplantation* Pregnancy Rats Rats, Brattleboro Rats, Inbred Strains Supraoptic Nucleus / transplantation* Vasopressins / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Neurophysins; 11000-17-2/Vasopressins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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