Document Detail


A printed nanolitre-scale bacterial sensor array.
MedLine Citation:
PMID:  20978670     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The last decade has witnessed a significant increase in interest in whole-cell biosensors for diverse applications, as well as a rapid and continuous expansion of array technologies. The combination of these two disciplines has yielded the notion of whole-cell array biosensors. We present a potential manifestation of this idea by describing the printing of a whole-cell bacterial bioreporters array. Exploiting natural bacterial tendency to adhere to positively charged abiotic surfaces, we describe immobilization and patterning of bacterial "spots" in the nanolitre volume range by a non-contact robotic printer. We show that the printed Escherichia coli-based sensor bacteria are immobilized on the surface, and retain their viability and biosensing activity for at least 2 months when kept at 4 °C. Immobilization efficiency was improved by manipulating the bacterial genetics (overproducing curli protein), the growth and the printing media (osmotic stress and osmoprotectants) and by a chemical modification of the inanimate surface (self-assembled layers of 3-aminopropyl-triethoxysilane). We suggest that the methodology presented herein may be applicable to the manufacturing of whole-cell sensor arrays for diverse high throughput applications.
Authors:
Sahar Melamed; Laura Ceriotti; Wilfried Weigel; François Rossi; Pascal Colpo; Shimshon Belkin
Publication Detail:
Type:  Journal Article     Date:  2010-10-26
Journal Detail:
Title:  Lab on a chip     Volume:  11     ISSN:  1473-0197     ISO Abbreviation:  Lab Chip     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-14     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128948     Medline TA:  Lab Chip     Country:  England    
Other Details:
Languages:  eng     Pagination:  139-46     Citation Subset:  IM    
Affiliation:
Department of Plant and Environmental Sciences, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel. shimshon@vms.huji.ac.il.
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