| A primer on using pooled shRNA libraries for functional genomic screens. | |
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MedLine Citation:
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PMID: 22271906 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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The discovery of RNA interference (RNAi) has revolutionized genetic analysis in mammalian cells. Loss-of-function RNAi screens enable rapid, functional annotation of the genome. Of the various RNAi approaches, pooled shRNA libraries have received considerable attention because of their versatility. A number of genome-wide shRNA libraries have been constructed against the human and mouse genomes, and these libraries can be readily applied to a variety of screens to interrogate the function of human and mouse genes in an unbiased fashion. We provide an introduction to the technical aspects of using pooled shRNA libraries for genetic screens. |
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Authors:
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Guang Hu; Ji Luo |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta biochimica et biophysica Sinica Volume: 44 ISSN: 1745-7270 ISO Abbreviation: Acta Biochim. Biophys. Sin. (Shanghai) Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-24 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101206716 Medline TA: Acta Biochim Biophys Sin (Shanghai) Country: China |
Other Details:
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Languages: eng Pagination: 103-12 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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