Document Detail


The primary structure of the precursor to core protein II, a putative member of mitochondrial processing protease family, of rat mitochondrial cytochrome bc1 complex deduced from cDNA sequence analysis.
MedLine Citation:
PMID:  2162168     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Core protein II of mitochondrial cytochrome bc1 complex is the second largest nuclear-encoded subunit. We have isolated two overlapping cDNA clones encoding the precursor to this protein by screening a rat liver cDNA library, and determined the nucleotide sequence of the cDNAs. Based on the deduced amino acid sequence of the corresponding human precursor, the mature polypeptide of rat core protein II appears to consist of 438 amino acid residues with a molecular weight of 46,751, and to be formed as a precursor with an amino-terminal presequence. The mature form of the rat protein shows 83% and at most 24% homology with its human and yeast counterparts, respectively. Northern blot analysis showed that rat liver poly(A)+ RNA contains a single mRNA of approximately 2000 nucleotides for the core protein II.
Authors:
Y Hosokawa; H Suzuki; H Toda; M Nishikimi; T Ozawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemistry international     Volume:  20     ISSN:  0158-5231     ISO Abbreviation:  Biochem. Int.     Publication Date:  1990  
Date Detail:
Created Date:  1990-07-16     Completed Date:  1990-07-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100311     Medline TA:  Biochem Int     Country:  AUSTRALIA    
Other Details:
Languages:  eng     Pagination:  731-7     Citation Subset:  IM    
Affiliation:
Department of Biomedical Chemistry, Faculty of Medicine, University of Nagoya, Japan.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
DNA / genetics,  isolation & purification
Electron Transport Complex III / genetics*
Humans
Mitochondria / enzymology*
Molecular Sequence Data
Molecular Weight
Protein Precursors / genetics*
Rats
Restriction Mapping
Sequence Homology, Nucleic Acid
Chemical
Reg. No./Substance:
0/Protein Precursors; 9007-49-2/DNA; EC 1.10.2.2/Electron Transport Complex III

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