| The primary structure identification of a corn peptide facilitating alcohol metabolism by HPLC-MS/MS. | |
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MedLine Citation:
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PMID: 22789607 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The aim of this study is to identify the primary structure of corn peptides (CPs) with a facilitating alcohol metabolism effect. Corn protein was hydrolyzed by Alcalase first. The hydrolysate, crude corn peptides (CPs), was then fractionated through ultrafiltration technology. The primary structure of a peptide from the fraction (Mm<5kDa) was identified by HPLC- MS/MS, coupled with the peptide sequence retrieval using the MS-MS online database. The amino acid sequence of the peptide was determined as Q-L-L-P-F, and the pentapeptide was synthesized by Fmoc solid-phase peptide synthesis (SPPS) method. Its ability to facilitate alcohol metabolism was evaluated in vivo. Results showed that the synthetic peptide (10mg/kg) had a higher ability to eliminate alcohol in vivo compared to the mixed peptides (Mm<5kDa, 200mg/kg). In conclusion, the pentapeptide Q-L-L-P-F has a potent ability in facilitating alcohol metabolism, and this pentapeptide is the main bioactive component in the mixed peptides obtained from corn. |
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Authors:
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Zhi-Li Ma; Wen-Jun Zhang; Guo-Cai Yu; Hui He; Yan Zhang |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-9 |
Journal Detail:
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Title: Peptides Volume: - ISSN: 1873-5169 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-13 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008690 Medline TA: Peptides Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2012. Published by Elsevier Inc. |
Affiliation:
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College of Food Science and Technology, Huazhong Agricultural University & Key Laboratory of Environment Correlative Dietology, Ministry of Education. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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