Document Detail

The preventive effect of low molecular weight heparin on CCL₄-induced necrosis and apoptosis in rat liver.
MedLine Citation:
PMID:  21057164     Owner:  NLM     Status:  MEDLINE    
INTRODUCTION: Heparin having anti-inflammatory and anti-fibrotic properties may have therapeutic effect on liver injury. The present study investigated the effect of low molecular weight heparin (Enoxaparin) on carbon tetrachloride (CCl₄) induced hepatic necrosis and apoptosis in rats.
MATERIAL AND METHODS: Thirty male rats were divided into 5 groups. Group I: Control; Group II: Olive oil dissolved CCl₄ at dose of 1 mL/kg, ip, twice per week; Group III: CCl₄ and Enoxaparin at dose of 180 IU/kg, sc, daily; Group IV: Enoxaparin; Group V: Olive oil at dose of 1 mL, ip, twice per week. The liver histology at the forth week was examined by haematoxylin-eosin, Masson.s trichrome, Toluidine blue and Periodic acid schiff stains. Proliferative and apoptotic activities were assessed semi-quantitatively by proliferating cell nuclear antigen (PCNA) and caspase-3 immune staining and TUNEL method. Semi-quantitative values formulated by the equation HSCORE =ΣP(i) (i+1) including both distribution and intensity of staining. Additionally, nidogen and a-smooth muscle actin were labeled by immunohistochemistry.
RESULTS: CCl₄ group had marked hepatocelluar necrosis around the vena centralis and increased inflammatory cells and mast cells. Hepatocytes showed deposition of lipid droplets, decrease in glycogen, apoptosis, and picnotic or enlarged nuclei. Enoxaparin reduced necrosis, apoptosis, and number of mast cells but had no effect on lipid droplets in hepatocytes. HSCORE.s of caspase-3 and PCNA were also significantly decreased by administration.
CONCLUSION: Enoxaparin have beneficial effects against necrosis as well as apoptosis at the early stage of CCL₄ induced liver injury.
Aysel Kukner; Fatma Tore; Tülin Firat; E Hakan Terzi; Hakan Oner; Yasemin H Balaban; Candan Ozogul
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of hepatology     Volume:  9     ISSN:  1665-2681     ISO Abbreviation:  Ann Hepatol     Publication Date:    2010 Oct-Dec
Date Detail:
Created Date:  2010-11-08     Completed Date:  2011-02-17     Revised Date:  2013-05-16    
Medline Journal Info:
Nlm Unique ID:  101155885     Medline TA:  Ann Hepatol     Country:  Mexico    
Other Details:
Languages:  eng     Pagination:  445-54     Citation Subset:  IM    
Abant Izzet Baysal University, Department of Histology and Embryology, Bolu, Turkey.
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MeSH Terms
Actins / metabolism
Apoptosis / drug effects*
Carbon Tetrachloride / adverse effects*,  pharmacology*
Caspase 3 / metabolism
Collagen / metabolism
Disease Models, Animal
Drug-Induced Liver Injury / pathology,  prevention & control*
Fibrinolytic Agents / therapeutic use*
Heparin, Low-Molecular-Weight / therapeutic use*
Liver / drug effects,  metabolism,  pathology*
Membrane Glycoproteins / metabolism
Necrosis / chemically induced,  prevention & control
Proliferating Cell Nuclear Antigen / metabolism
Rats, Sprague-Dawley
Reg. No./Substance:
0/Actins; 0/Fibrinolytic Agents; 0/Heparin, Low-Molecular-Weight; 0/Membrane Glycoproteins; 0/Proliferating Cell Nuclear Antigen; 0/nidogen; 56-23-5/Carbon Tetrachloride; 9007-34-5/Collagen; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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