| The pressure-induced, reversible inactivation of mouse brain enolases. | |
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MedLine Citation:
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PMID: 7151797 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The three isozymes of mouse brain enolase (alpha alpha, alpha gamma and gamma gamma) were subjected to pressures between 160 MPa and 380 MPa. Enzymatic activity 2 min after the pressure exposure was used to monitor pressure-induced changes in the physical state of the protein. Pressure caused a reversible inactivation of all three forms; alpha alpha showed a continual inactivation with increasing pressure whereas the inactivation curves for alpha gamma and gamma gamma were biphasic. P1 2 for alpha alpha was 190 MPa, for alpha gamma 210 MPa and for gamma gamma 280 MPa. Volume changes associated with this inactivation were calculated for all three forms of the enzyme; delta V0 varied from -150 ml mol-1 to -170 ml mol-1, assuming a model in which there was no change in the aggregation state of the enzyme, or from -230 ml mol-1 to -250 ml mol-1 assuming that the enzyme dissociated on exposure to pressure. The half-time for reactivation was measured as a function of the concentration of enzyme; the results are consistent with an inactivation that is due to dissociation of the dimeric enzyme into monomers. |
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Authors:
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J Kornblatt; J Kornblatt; G Hui Bon Hoa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of biochemistry / FEBS Volume: 128 ISSN: 0014-2956 ISO Abbreviation: Eur. J. Biochem. Publication Date: 1982 Nov |
Date Detail:
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Created Date: 1983-03-11 Completed Date: 1983-03-11 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 0107600 Medline TA: Eur J Biochem Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 577-81 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / enzymology* Kinetics Mice Phosphopyruvate Hydratase / metabolism* Pressure Thermodynamics |
| Chemical | |
Reg. No./Substance:
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EC 4.2.1.11/Phosphopyruvate Hydratase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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