| The preservation of urine samples for determination of renal stone risk factors. | |
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MedLine Citation:
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PMID: 11539109 Owner: NASA Status: MEDLINE |
Abstract/OtherAbstract:
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A preservation technique for urine specimens before determination of stone risk factors was evaluated. The purpose of these experiments was to prove the effectiveness of the preservatives used to prevent changes in the concentrations of those constituents measured. Measured concentrations in fresh specimens were compared with those in the same specimens after storage with the preservatives. Refrigeration at 4 degrees C up to five days was appropriate in a laboratory setting, as no significant changes in urinary concentrations occurred. Refrigeration, however, did not offer a convenient method for shipping. Chemical preservation was found to be an effective alternative to refrigeration. Thymol prevented changes in concentration of pH, citrate, uric acid, sulfate, sodium, potassium, and cyclic AMP, while a mixture of hydrochloric (HCl) acid and boric acid prevented changes in calcium, magnesium, phosphorus, oxalate, ammonium, and creatinine. Thus, the addition of thymol or HCl/boric acid to urine specimens will prevent significant changes in the concentrations of stone risk factors. |
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Authors:
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M J Nicar; M C Hsu; T Johnson; C Y Pak |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Laboratory medicine Volume: 18 ISSN: 0007-5027 ISO Abbreviation: Lab Med Publication Date: 1987 Jun |
Date Detail:
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Created Date: 1996-12-16 Completed Date: 1996-12-16 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0250641 Medline TA: Lab Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 382-4 Citation Subset: S |
Affiliation:
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Center on Mineral Metabolism and Clinical Research, Southwestern Medical School of the University of Texas Health Science Center at Dallas, TX 75235, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Boric Acids
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pharmacology Humans Hydrochloric Acid / pharmacology Kidney Calculi / diagnosis* Preservation, Biological / methods* Preservatives, Pharmaceutical / pharmacology* Refrigeration Risk Factors Specimen Handling / methods* Thymol / pharmacology Urinalysis |
| Chemical | |
Reg. No./Substance:
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0/Boric Acids; 0/Preservatives, Pharmaceutical; 11113-50-1/boric acid; 7647-01-0/Hydrochloric Acid; 89-83-8/Thymol |
| Investigator | |
Investigator/Affiliation:
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C Y Pak / U TX Health Science Center , Dallas |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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