Document Detail

The preservation of urine samples for determination of renal stone risk factors.
MedLine Citation:
PMID:  11539109     Owner:  NASA     Status:  MEDLINE    
A preservation technique for urine specimens before determination of stone risk factors was evaluated. The purpose of these experiments was to prove the effectiveness of the preservatives used to prevent changes in the concentrations of those constituents measured. Measured concentrations in fresh specimens were compared with those in the same specimens after storage with the preservatives. Refrigeration at 4 degrees C up to five days was appropriate in a laboratory setting, as no significant changes in urinary concentrations occurred. Refrigeration, however, did not offer a convenient method for shipping. Chemical preservation was found to be an effective alternative to refrigeration. Thymol prevented changes in concentration of pH, citrate, uric acid, sulfate, sodium, potassium, and cyclic AMP, while a mixture of hydrochloric (HCl) acid and boric acid prevented changes in calcium, magnesium, phosphorus, oxalate, ammonium, and creatinine. Thus, the addition of thymol or HCl/boric acid to urine specimens will prevent significant changes in the concentrations of stone risk factors.
M J Nicar; M C Hsu; T Johnson; C Y Pak
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Laboratory medicine     Volume:  18     ISSN:  0007-5027     ISO Abbreviation:  Lab Med     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1996-12-16     Completed Date:  1996-12-16     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0250641     Medline TA:  Lab Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  382-4     Citation Subset:  S    
Center on Mineral Metabolism and Clinical Research, Southwestern Medical School of the University of Texas Health Science Center at Dallas, TX 75235, USA.
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MeSH Terms
Boric Acids / pharmacology
Hydrochloric Acid / pharmacology
Kidney Calculi / diagnosis*
Preservation, Biological / methods*
Preservatives, Pharmaceutical / pharmacology*
Risk Factors
Specimen Handling / methods*
Thymol / pharmacology
Reg. No./Substance:
0/Boric Acids; 0/Preservatives, Pharmaceutical; 11113-50-1/boric acid; 7647-01-0/Hydrochloric Acid; 89-83-8/Thymol
C Y Pak / U TX Health Science Center , Dallas

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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