Document Detail


The presence of professional phagocytes dictates the number of host cells targeted for Yop translocation during infection.
MedLine Citation:
PMID:  20148898     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Type III secretion systems deliver effector proteins from Gram-negative bacterial pathogens into host cells, where they disarm host defences, allowing the pathogens to establish infection. Although Yersinia pseudotuberculosis delivers its effector proteins, called Yops, into numerous cell types grown in culture, we show that during infection Y. pseudotuberculosis selectively targets Yops to professional phagocytes in Peyer's patches, mesenteric lymph nodes and spleen, although it colocalizes with B and T cells as well as professional phagocytes. Strikingly, in the absence of neutrophils, the number of cells with translocated Yops was significantly reduced although the bacterial loads were similar, indicating that Y. pseudotuberculosis did not arbitrarily deliver Yops to the available cells. Using isolated splenocytes, selective binding and selective targeting to professional phagocytes when bacteria were limiting was also observed, indicating that tissue architecture was not required for the tropism for professional phagocytes. In isolated splenocytes, YadA and Invasin increased the number of all cells types with translocated Yops, but professional phagocytes were still preferentially translocated with Yops in the absence of these adhesins. Together these results indicate that Y. pseudotuberculosis discriminates among cells it encounters during infection and selectively delivers Yops to phagocytes while refraining from translocation to other cell types.
Authors:
Enrique A Durand; Francisco J Maldonado-Arocho; Cynthia Castillo; Rebecca L Walsh; Joan Mecsas
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-02-09
Journal Detail:
Title:  Cellular microbiology     Volume:  12     ISSN:  1462-5822     ISO Abbreviation:  Cell. Microbiol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-19     Completed Date:  2010-10-14     Revised Date:  2014-06-25    
Medline Journal Info:
Nlm Unique ID:  100883691     Medline TA:  Cell Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1064-82     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Bacterial Proteins / metabolism*
Lymph Nodes / immunology,  microbiology
Mice
Mice, Inbred BALB C
Peyer's Patches / immunology,  microbiology
Phagocytes / immunology*,  microbiology*
Protein Transport
Spleen / immunology,  microbiology
Virulence Factors / metabolism*
Yersinia Infections / immunology*,  microbiology
Yersinia pseudotuberculosis / immunology*,  pathogenicity*
Grant Support
ID/Acronym/Agency:
5K12GM074869/GM/NIGMS NIH HHS; AI056068/AI/NIAID NIH HHS; K12 GM074869/GM/NIGMS NIH HHS; K12 GM074869-04/GM/NIGMS NIH HHS; R01 AG019781-10/AG/NIA NIH HHS; R01 AI056068-05/AI/NIAID NIH HHS; R25 GM066567-06/GM/NIGMS NIH HHS; R25GM066567/GM/NIGMS NIH HHS; R56 AI073759-01A2/AI/NIAID NIH HHS; T32 AI007422-18/AI/NIAID NIH HHS; T32 GM007310/GM/NIGMS NIH HHS; T32 GM007310-32/GM/NIGMS NIH HHS; T32AI007422/AI/NIAID NIH HHS; T32GM07310/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Virulence Factors
Comments/Corrections

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