Document Detail

The presence of pregnancy-associated plasma protein-A in human corpora lutea: cellular and subcellular distribution and dependence on reproductive state.
MedLine Citation:
PMID:  1707688     Owner:  NLM     Status:  MEDLINE    
Pregnancy-associated plasma protein-A (PAPP-A) is a high-molecular-weight glycoprotein primarily secreted by syncytiotrophoblasts of human placenta. It is not known, however, whether human CL of menstrual cycle or pregnancy also contain this protein. Therefore, light and electron microscope immunocytochemical studies were undertaken to investigate the presence, cellular and subcellular distribution, and dependence of luteal PAPP-A content on reproductive state. Human CL from early, mid, and late luteal phases and from term pregnancies immunostained specifically for PAPP-A. Immunostaining was found in large luteal cells (17-30 microns) but not in small luteal cells (7-16 microns) or in nonluteal cells in any of the reproductive states. Immunostaining was not found in negative control tissues, i.e. human liver or bovine CL of pregnancy. As expected however, term-pregnancy human placenta used for a positive control tissue immunostained intensely for PAPP-A. The luteal immunostaining was highest in early luteal phase, decreased progressively from early to mid and from mid to late luteal phases, and then disappeared in corpora albicantia. The relative intensity of immunostaining in early luteal phase human CL was similar to that in term-pregnancy human placenta and higher than in term-pregnancy human CL. The immunogold particles due to PAPP-A were primarily associated with secretory granules of large luteal cells. A small number of gold particles were also found in rough endoplasmic reticulum and cytoplasm. In conclusion, human CL contain immunoreactive PAPP-A. The luteal content varies with reproductive state, with the highest amount found in early luteal phase CL.(ABSTRACT TRUNCATED AT 250 WORDS)
N Chegini; Z M Lei; C V Rao; P Bischof
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biology of reproduction     Volume:  44     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1991 Jan 
Date Detail:
Created Date:  1991-05-20     Completed Date:  1991-05-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  201-6     Citation Subset:  IM    
Department of Obstetrics and Gynecology, University of Louisville School of Medicine, Kentucky 40292.
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MeSH Terms
Corpus Luteum / metabolism*
Luteal Phase / physiology
Microscopy, Immunoelectron
Placenta / metabolism
Pregnancy-Associated Plasma Protein-A / metabolism*
Reproduction / physiology
Subcellular Fractions / metabolism
Grant Support
Reg. No./Substance:
EC 3.4.24.-/Pregnancy-Associated Plasma Protein-A

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