Document Detail


The presence of Merkel cell polyomavirus is associated with deregulated expression of BRAF and Bcl-2 genes in non-small cell lung cancer.
MedLine Citation:
PMID:  23355004     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Polyomaviruses such as BK virus (BKV), JC virus (JCV) and Merkel cell polyomavirus (MCPyV) are typically nononcogenic, although they have been detected in a variety of human neoplasms. The aim of our study was to determine the frequency of the most common polyomaviruses MCPyV, BKV and JCV as well as the gene expression profile of genes involved in oncogenesis including K-ras, BRAF, RKIP, Bax, Bcl-2, p53 and RB1 in a cohort of non-small cell lung cancer (NSCLC) patients. Real-time and nested polymerase chain reaction (PCR) were used to assess the presence of polyomaviruses DNA in tissue biopsies from 110 patients with primary NSCLC and 14 tissue specimens from macroscopically healthy sites of their lung. Real-time PCR was also used to determine the mRNA expression of K-ras, BRAF, RKIP, Bax, Bcl-2, p53 and RB1 in selected samples. Results showed that ten NSCLC specimens were positive for the presence of MCPyV DNA (10/110, 9.1%), whereas no control sample was tested positive for the virus. The MCPyV-positive samples were predominantly obtained from male smokers (9/10). BKV and JCV DNA were not detected either in lung tissues biopsies or the control specimens. Interestingly, gene expression analysis revealed increased mRNA and protein expression of BRAF gene in association with BRAF phosphorylation in the MCPyV-positive samples, whereas Bcl-2 gene expression was downregulated in the same type of samples. The detected MCPyV prevalence in NSCLC in combination with the deregulated expression of BRAF and Bcl-2 genes suggests that these events are likely to contribute to the pathogenesis of NSCLC.
Authors:
I Lasithiotaki; K M Antoniou; S P Derdas; E Sarchianaki; E K Symvoulakis; A Psaraki; D A Spandidos; E N Stathopoulos; N M Siafakas; G Sourvinos
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Publication Detail:
Type:  Journal Article     Date:  2013-02-27
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  133     ISSN:  1097-0215     ISO Abbreviation:  Int. J. Cancer     Publication Date:  2013 Aug 
Date Detail:
Created Date:  2013-05-22     Completed Date:  2013-07-25     Revised Date:  2013-11-22    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  604-11     Citation Subset:  IM    
Copyright Information:
Copyright © 2013 UICC.
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MeSH Terms
Descriptor/Qualifier:
Aged
Carcinoma, Non-Small-Cell Lung / genetics*,  virology
DNA, Viral / genetics
Female
Humans
Lung Neoplasms / genetics*,  virology
Male
Merkel cell polyomavirus / immunology*,  isolation & purification
Middle Aged
Phosphatidylethanolamine Binding Protein / genetics
Polyomavirus Infections / genetics,  virology
Proto-Oncogene Proteins / genetics
Proto-Oncogene Proteins B-raf / genetics*,  metabolism
Proto-Oncogene Proteins c-bcl-2 / genetics*,  metabolism
RNA, Messenger / genetics,  metabolism
Smoking
Tumor Suppressor Protein p53 / genetics
Tumor Virus Infections / genetics
ras Proteins / genetics
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/KRAS protein, human; 0/PEBP1 protein, human; 0/Phosphatidylethanolamine Binding Protein; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/RNA, Messenger; 0/Tumor Suppressor Protein p53; EC 2.7.11.1/BRAF protein, human; EC 2.7.11.1/Proto-Oncogene Proteins B-raf; EC 3.6.5.2/ras Proteins
Comments/Corrections
Comment In:
Int J Cancer. 2013 Dec 15;133(12):3016-7   [PMID:  23740689 ]
Int J Cancer. 2013 Dec 15;133(12):3014-5   [PMID:  23740604 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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