Document Detail

The presence of interleukin-1beta in the bovine reproductive tract.
MedLine Citation:
PMID:  10213467     Owner:  NLM     Status:  MEDLINE    
Interleukin-1 (IL-1) is a pleiotropic cytokine implicated in endometrial and embryonic physiology. Our objective was to determine the presence of IL-1 in the endometrium, oviduct, and uterine fluid of cows at days 0, 7, and 14 of the estrous cycle. Immunoreactive IL-1beta was identified in endometrial and oviductal tissues throughout the estrous cycle by immunohistochemistry. Both glandular and luminal endometrial epithelium exhibited intense IL-1beta staining. For luminal epithelium, staining was strongest at day 0 and least at day 7. Staining in glandular epithelium was similar at all stages of the estrous cycle examined. There was a diffuse immunostaining throughout the endometrial stroma, and some isolated stromal cells stained strongly, as did endothelial cells. Immunoreactive IL-1beta was detected in uterine flushings by Western blotting, and the frequency of positive samples and intensity of immunoreactive bands did not differ between days of the estrous cycle. In the oviduct, immunoreactive IL-1beta was found in the epithelium and stroma of ampulla and isthmus. The staining intensity score for the oviduct was not different between isthmus and ampulla or between days of the estrous cycle. The presence of IL-1beta in the bovine endometrium, oviduct, and uterine flushings supports the idea that this cytokine may play an important role in regulating embryonic and endometrial function in cattle.
F F Paula-Lopes; A A de Moraes; P J Hansen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research     Volume:  19     ISSN:  1079-9907     ISO Abbreviation:  J. Interferon Cytokine Res.     Publication Date:  1999 Mar 
Date Detail:
Created Date:  1999-06-15     Completed Date:  1999-06-15     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9507088     Medline TA:  J Interferon Cytokine Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  279-85     Citation Subset:  IM    
Department of Dairy and Poultry Sciences, University of Florida, Gainesville 32611-0920, USA.
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MeSH Terms
Blotting, Western
Body Fluids / chemistry*
Cells, Cultured
Endometrium / chemistry*
Estrus / physiology
Fallopian Tubes / chemistry*
Interleukin-1 / analysis*
Recombinant Proteins / analysis
Uterus / chemistry*
Reg. No./Substance:
0/Interleukin-1; 0/Recombinant Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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