| The presence of fatty acids in human alpha-fetoprotein. | |
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MedLine Citation:
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PMID: 75886 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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alpha-Fetoprotein has been prepared from human fetal tissue by procedures utilizing DEAE-Sephadex, concanavalin A-Sepharose, and isoelectric focusing. A major and a minor component with isoelectric points of 4.7 and 5.3, respectively, have been isolated and are similar to those prepared under various conditions by other investigators. The 4.7 material contains 2.4 mol of fatty acids/mol of protein, whereas the minor component is fat-free. The relative amounts of fatty acid vary somewhat with different preparations. The ranges found in three isolates were as follows: palmitic acid (8 to 11%), stearic acid (2 to 5%), oleic acid (10 to 28%), linoleic acid (7 to 15%), arachidonic acid (12 to 39%), and 4,7,10,13,16,19-docosahexaenoic acid (16 to 42%). Human fetal serum albumin contained 0.7 mol of fatty acid/mol of protein, with arachidonic acid and the docosahexaenoic acid comprising only 11.4% of the total. Removal of fatty acids by treatment with charcoal converted alpha-fetoprotein into material with an isoelectric point of pH 5.3. Addition of arachidonic acid to the lipid-free protein restored it to protein with a pH 4.7 isoelectric point, typical of the major native component. The possible role of the fatty acids in alpha-fetoprotein on the inhibition of various lymphocyte functions is projected. |
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Authors:
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D C Parmelee; M A Evenson; H F Deutsch |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 253 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1978 Apr |
Date Detail:
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Created Date: 1978-05-24 Completed Date: 1978-05-24 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2114-9 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Gas Fatty Acids / analysis Female Fetus Humans Pregnancy alpha-Fetoproteins* / isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 0/alpha-Fetoproteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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