Document Detail


The presence of amino acids affects inorganic N uptake in non-mycorrhizal seedlings of European beech (Fagus sylvatica).
MedLine Citation:
PMID:  20595637     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To investigate the impact of organic N compounds for inorganic nitrogen uptake in the rhizosphere, we fed ammonium nitrate with or without amino acids (i.e., glutamine or arginine) to the roots of non-mycorrhizal beech (Fagus sylvatica L.) seedlings under controlled conditions at different levels of N availability. Uptake of individual N sources was determined from ¹⁵N (inorganic N) and ¹⁵N ¹³C (organic N) accumulation in the roots. In addition, gene fragments encoding proteins involved in N uptake and metabolism were cloned from beech for gene expression analyses by quantitative real-time PCR in the roots. Generally, ammonium was preferred over nitrate as N source. Organic N sources were taken up by beech roots as intact molecules. Uptake of organic N was significantly higher than inorganic N uptake, thus contributing significantly to N nutrition of beech. Depending on the level of N availability, inorganic N uptake was negatively affected by the presence of organic N sources. This result indicates an overestimation of the contribution of inorganic N uptake to N nutrition of beech in previous studies. Apparently, association with mycorrhizal fungi is not essential for organic N uptake by beech roots. Gene expression analyses showed that transcriptional regulation of the amino acid transporters FsCAT3, FsCAT5, FsAAT and FsAAP and the ammonium transporter FsAMT1.2 in the roots is involved in N nutrition of beech.
Authors:
Gunda Stoelken; Judy Simon; Barbara Ehlting; Heinz Rennenberg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-01
Journal Detail:
Title:  Tree physiology     Volume:  30     ISSN:  0829-318X     ISO Abbreviation:  Tree Physiol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-08-10     Completed Date:  2010-11-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100955338     Medline TA:  Tree Physiol     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  1118-28     Citation Subset:  IM    
Affiliation:
Institute of Forest Botany and Tree Physiology, Chair of Tree Physiology, University of Freiburg, Georges-Koehler-Allee 53/54, 79110 Freiburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / chemistry,  metabolism*
Fagus / metabolism*,  microbiology
Gene Expression Regulation, Plant / physiology
Mycorrhizae / physiology*
Nitrogen / chemistry,  metabolism*
Seedling / metabolism*
Soil / analysis
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Soil; 7727-37-9/Nitrogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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