| The prepattern transcription factor Irx3 directs nephron segment identity. | |
| | |
MedLine Citation:
|
PMID: 17875669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The nephron, the basic structural and functional unit of the vertebrate kidney, is organized into discrete segments, which are composed of distinct renal epithelial cell types. Each cell type carries out highly specific physiological functions to regulate fluid balance, osmolarity, and metabolic waste excretion. To date, the genetic basis of regionalization of the nephron has remained largely unknown. Here we show that Irx3, a member of the Iroquois (Irx) gene family, acts as a master regulator of intermediate tubule fate. Comparative studies in Xenopus and mouse have identified Irx1, Irx2, and Irx3 as an evolutionary conserved subset of Irx genes, whose expression represents the earliest manifestation of intermediate compartment patterning in the developing vertebrate nephron discovered to date. Intermediate tubule progenitors will give rise to epithelia of Henle's loop in mammals. Loss-of-function studies indicate that irx1 and irx2 are dispensable, whereas irx3 is necessary for intermediate tubule formation in Xenopus. Furthermore, we demonstrate that misexpression of irx3 is sufficient to direct ectopic development of intermediate tubules in the Xenopus mesoderm. Taken together, irx3 is the first gene known to be necessary and sufficient to specify nephron segment fate in vivo. |
| | |
Authors:
|
Luca Reggiani; Daniela Raciti; Rannar Airik; Andreas Kispert; André W Brändli |
Related Documents
:
|
19164599 - Gene expression profile of the third pharyngeal pouch reveals role of mesenchymal mafb ... 9053309 - The one-eyed pinhead gene functions in mesoderm and endoderm formation in zebrafish and... 12112459 - Efficient ectopic gene expression targeting chick mesoderm. 18381349 - Genetic interaction between bardet-biedl syndrome genes and implications for limb patte... 21384259 - Atherosclerosis: an epigenetic balancing act that goes wrong. 9186059 - Backfoot is a novel homeobox gene expressed in the mesenchyme of developing hind limb. 16201299 - Cationic liposome-mediated bcl-xl gene transfection into human keratocytes. 10759509 - The expression of d-cyclin genes defines distinct developmental zones in snapdragon api... 12754939 - Hardware (dna) circuits. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Genes & development Volume: 21 ISSN: 0890-9369 ISO Abbreviation: Genes Dev. Publication Date: 2007 Sep |
Date Detail:
|
Created Date: 2007-09-18 Completed Date: 2007-10-25 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 8711660 Medline TA: Genes Dev Country: United States |
Other Details:
|
Languages: eng Pagination: 2358-70 Citation Subset: IM |
Affiliation:
|
Department of Chemistry and Applied Biosciences, Institute of Pharmaceutical Sciences, ETH Zurich, CH-8093, Zurich, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Body Patterning / genetics* Embryo, Nonmammalian Gene Expression Regulation, Developmental Homeodomain Proteins / physiology* Kidney Tubules / embryology Mice Models, Biological Nephrons / embryology* Transcription Factors / physiology* Xenopus / embryology*, genetics |
| Chemical | |
Reg. No./Substance:
|
0/Homeodomain Proteins; 0/Transcription Factors; 0/Xiro3 protein, Xenopus |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: N-cofilin is associated with neuronal migration disorders and cell cycle control in the cerebral cor...
Next Document: In vitro glucuronidation of thyroxine and triiodothyronine by liver microsomes and recombinant human...