Document Detail

The preparation and characterisation of native photoreceptor units from the thylakoids of Rhodopseudomonas viridis.
MedLine Citation:
PMID:  16453514     Owner:  NLM     Status:  PubMed-not-MEDLINE    
The photosynthetic membranes of Rhodopseudomonas viridis consist of a regular array of structural units. Each unit is composed of a central core (thought to contain the reaction centre complex) surrounded by a subdivided ring of protein (of likely antennae function). These individual units can be dissociated from the membrances using a variety of detergent treatments. The absorption spectrum, used as a criterion of a native state, is retained. All of the seven major polypeptides, the four reaction centre polypeptides (cytochrome, H, M and L chain) as well as the three light-harvesting polypeptides (B1015-alpha, beta and xi) are shown to be present. Electron microscopy of the units shows a similar structure to the units within the membrane. surface-specific iodination of both membranes and units labels predominantly polypeptides H, B1015-alpha, and xi. M and L are weakly labelled. In addition, B1015-beta is labelled in the isolated units. This, with other evidence, supports an allocation of light-harvesting polypeptides to the outer ring. Further solubilisation of these units separates the reaction centre (as a native complex containing all four polypeptides) from the light-harvesting polypeptides. The light-harvesting polypeptides are obtained in a form containing all three polypeptides and bound pigment, however the peak at 1015 nm corresponding to native bacteriochlorophyll b is lost.
F Jay; M Lambillotte; W Stark; K Mühlethaler
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The EMBO journal     Volume:  3     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1984 Apr 
Date Detail:
Created Date:  2010-06-28     Completed Date:  2010-06-28     Revised Date:  2010-09-20    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  England    
Other Details:
Languages:  eng     Pagination:  773-6     Citation Subset:  -    
Institute for Cell Biology, Federal Institute of Technology, CH-8093 Zürich, Switzerland.
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