| The preimplantation pig embryo: cell number and allocation to trophectoderm and inner cell mass of the blastocyst in vivo and in vitro. | |
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MedLine Citation:
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PMID: 3168789 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Total cell number as well as differential cell numbers representing the inner cell mass (ICM) and trophectoderm were determined by a differential staining technique for preimplantation pig embryos recovered between 5 and 8 days after the onset of oestrus. Total cell number increased rapidly over this time span and significant effects were found between embryos of the same chronological age from different females. Inner cells could be detected in some but not all embryos of 12-16 cells. The proportion of inner cells was low in morulae but increased during differentiation of ICM and trophectoderm in early blastocysts. The proportion of ICM cells then decreased as blastocysts expanded and hatched. Some embryos were cultured in vitro and others were transferred to the oviducts of immature mice as a surrogate in vivo environment and assessed for morphology and cell number after several days. Although total cell number did not reach in vivo levels, morphological development and cell number increase was sustained better in the immature mice than in vitro. The proportion of ICM cells in blastocysts formed in vitro was in the normal range. |
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Authors:
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V E Papaioannou; K M Ebert |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 102 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 1988 Apr |
Date Detail:
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Created Date: 1988-11-15 Completed Date: 1988-11-15 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 793-803 Citation Subset: IM |
Affiliation:
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Department of Pathology, Tufts University School of Medicine, Boston, MA 02111. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / cytology* Cell Count Cells, Cultured Embryo, Mammalian / cytology Embryonic Development* Female Immune Sera Microscopy, Fluorescence Morula / cytology Pregnancy Swine Time Factors |
| Grant Support | |
ID/Acronym/Agency:
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5 RO1 HD21616/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Immune Sera |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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