Document Detail


A predictive model of cell traction forces based on cell geometry.
MedLine Citation:
PMID:  21044567     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent work has indicated that the shape and size of a cell can influence how a cell spreads, develops focal adhesions, and exerts forces on the substrate. However, it is unclear how cell shape regulates these events. Here we present a computational model that uses cell shape to predict the magnitude and direction of forces generated by cells. The predicted results are compared to experimentally measured traction forces, and show that the model can predict traction force direction, relative magnitude, and force distribution within the cell using only cell shape as an input. Analysis of the model shows that the magnitude and direction of the traction force at a given point is proportional to the first moment of area about that point in the cell, suggesting that contractile forces within the cell act on the entire cytoskeletal network as a single cohesive unit. Through this model, we demonstrate that intrinsic properties of cell shape can facilitate changes in traction force patterns, independently of heterogeneous mechanical properties or signaling events within the cell.
Authors:
Christopher A Lemmon; Lewis H Romer
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Publication Detail:
Type:  Letter; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biophysical journal     Volume:  99     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-11-03     Completed Date:  2011-01-28     Revised Date:  2011-11-03    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  L78-80     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.
Affiliation:
Department of Cell Biology, Duke University Medical Center, Durham, North Carolina, USA. christopher.lemmon@duke.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Biomechanics
Biophysical Phenomena
Cell Adhesion / physiology*
Cell Movement / physiology*
Cell Shape / physiology*
Cells, Cultured
Cytoskeleton / physiology
Mice
Models, Biological*
Signal Transduction
Grant Support
ID/Acronym/Agency:
GM089331/GM/NIGMS NIH HHS; HL088203/HL/NHLBI NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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