Document Detail


A predicted membrane protein, TRA-2A, directs hermaphrodite development in Caenorhabditis elegans.
MedLine Citation:
PMID:  7555725     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The nematode C. elegans naturally develops as either an XO male or XX hermaphrodite. The sex-determining gene, tra-2, promotes hermaphrodite development in XX animals. This gene encodes a predicted membrane protein, named TRA-2A, which has been proposed to provide the primary feminising activity of the tra-2 locus. Here, we show that transgenic TRA-2A driven from a heat shock promoter can fully feminise the somatic tissues of XX tra-2 loss-of-function mutants, which would otherwise develop as male. TRA-2A is thus likely to provide a component of the tra-2 locus that is both necessary and sufficient to promote female somatic development. Transgenic TRA-2A driven by the heat shock promoter can also transform XO animals from male to self-fertile hermaphrodite. This result establishes the role of tra-2 as a developmental switch that controls somatic sexual cell fate. We show that a carboxy-terminal region of TRA-2A, predicted to be intra-cellular, can partially feminise XX tra-2 loss-of-function mutants and XO tra-2(+) males. We suggest that this intra-cellular domain of TRA-2A promotes hermaphrodite development by negatively regulating the FEM proteins.
Authors:
P E Kuwabara; J Kimble
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  121     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1995 Sep 
Date Detail:
Created Date:  1995-11-08     Completed Date:  1995-11-08     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2995-3004     Citation Subset:  IM    
Affiliation:
Howard Hughes Medical Institute, University of Wisconsin, Madison 53706, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Genetically Modified
Caenorhabditis elegans / embryology*,  genetics
Drosophila Proteins*
Electrophoresis, Polyacrylamide Gel
Female
Genotype
Hermaphroditism / embryology*,  genetics
Hot Temperature
Male
Mutation
Phenotype
Ribonucleoproteins / genetics,  physiology*
Sex Differentiation*
Grant Support
ID/Acronym/Agency:
HD24663/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Ribonucleoproteins; 0/tra2 protein, Drosophila

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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